Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613125112_b3af AGAACTATACATAGTGAGACCATGTAACCCTG(+AGGACA)GATCCTAACAACACAGTCTGTAAATAAAAGT NGG 10 8 17 8 24 0 100 5 10
    240613125110_yqr5 AATAAACTTTTATTTACAGACTGTGTTGTTAG(+AGGACA)GATCCAGGGTTACATGGTCTCACTATGTATA NGG 10 8 17 8 24 0 100 5 10
    240613125109_o53f TAGGTGACATTCAGAGATGCTGTGTTGGGCTG(+AGGACA)GTACCAGAATGCATTGATGTGTGTGCTTTAC NGG 10 8 17 8 24 0 100 5 10
    240613125108_zjh9 CAACGGTAAAGCACACACATCAATGCATTCTG(+AGGACA)GTACCAGCCCAACACAGCATCTCTGAATGTC NGG 10 8 17 8 24 0 100 5 10
    240613125107_dy0h TATGCTACTTGCACTGTGACATGGCATTTGAG(+AGGACA)GATCCCTGACTTACAGACAAAGCTTAAAATG NGG 10 8 17 8 24 0 100 5 10
    240613125105_uze8 TGTGTCATTTTAAGCTTTGTCTGTAAGTCAGG(+AGGACA)GATCCTCAAATGCCATGTCACAGTGCAAGTA NGG 10 8 17 8 24 0 100 5 10
    240613125104_q72h AAAGCTCATCACAAGGTCTACTAAGCATTAAG(+AGGACA)GATCCTCATTATAAGTCCTCTGTACCACTTT NGG 10 8 17 8 24 0 100 5 10
    240613125102_y795 AAAAAAAAGTGGTACAGAGGACTTATAATGAG(+AGGACA)GATCCTTAATGCTTAGTAGACCTTGTGATGA NGG 10 8 17 8 24 0 100 5 10
    240613125101_koi9 AATACTGACTCTCATTAGATATGATCTTATAG(+AGGACA)GATCCATACCCTCCCTAGGACCAAATATATC NGG 10 8 17 8 24 0 100 5 10
    240613125100_eewt CATCAGATATATTTGGTCCTAGGGAGGGTATG(+AGGACA)GATCCTATAAGATCATATCTAATGAGAGTCA NGG 10 8 17 8 24 0 100 5 10