Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613125128_b426 GTCACATTGTATCCAGGCAGGAAGCAGAGATG(+AGGACA)GATCCAGGTGCTCGGTCCCCTCTCCTGTTCT NGG 10 8 17 8 24 0 100 5 10
    240613125126_u0sg CTATAAGAACAGGAGAGGGGACCGAGCACCTG(+AGGACA)GATCCATCTCTGCTTCCTGCCTGGATACAAT NGG 10 8 17 8 24 0 100 5 10
    240613125125_ypeg GTAAACACACAAACAGTAAGCTTAGCTGTTGG(+AGGACA)GATCCTGCCCCGAGGTCACCACTCCCTTCAT NGG 10 8 17 8 24 0 100 5 10
    240613125123_l2n0 ATGGAATGAAGGGAGTGGTGACCTCGGGGCAG(+AGGACA)GATCCCAACAGCTAAGCTTACTGTTTGTGTG NGG 10 8 17 8 24 0 100 5 10
    240613125122_4si9 GTGGGCCTTTTAAATGTCCTATGGGATACAAG(+AGGACA)GTACCAAGCCAGTGGAACCTGACAGAAATAT NGG 10 8 17 8 24 0 100 5 10
    240613125121_vk0a TTGAAATATTTCTGTCAGGTTCCACTGGCTTG(+AGGACA)GTACCTTGTATCCCATAGGACATTTAAAAGG NGG 10 8 17 8 24 0 100 5 10
    240613125120_qfyo TGCAGGAGGTTTGTCGCTGAAGCCATGCGATG(+AGGACA)GATCCAGAGAGACTTTTTAATAATCCTTAGG NGG 10 8 17 8 24 0 100 5 10
    240613125118_i4ux TATTACCTAAGGATTATTAAAAAGTCTCTCTG(+AGGACA)GATCCATCGCATGGCTTCAGCGACAAACCTC NGG 10 8 17 8 24 0 100 5 10
    240613125116_35k2 TCCTGTGGTTTTGTAAACAGAGATATTGGGGG(+AGGACA)GATCCTCCTTGAAATAGTTTGGAAGAATTGC NGG 10 8 17 8 24 0 100 5 10
    240613125115_4ls4 ACAGTGCAATTCTTCCAAACTATTTCAAGGAG(+AGGACA)GATCCCCCCAATATCTCTGTTTACAAAACCA NGG 10 8 17 8 24 0 100 5 10