Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613125225_evor TCGCTTGGAGGGTGGACAAGGGGCAGTCCTAG(+AGGACA)GTACCAGGCTCTCAGTCTCGAGCATCCCAGA NGG 10 8 17 8 24 0 100 5 10
    240613125224_x0wm CGGTGTCTGGGATGCTCGAGACTGAGAGCCTG(+AGGACA)GTACCTAGGACTGCCCCTTGTCCACCCTCCA NGG 10 8 17 8 24 0 100 5 10
    240613125222_ekgg CAGACCTGGAAGTCCTCTAGTGTATGTGTTAG(+AGGACA)GATCCTCATATCAGCTGATGTATGCTGTTTG NGG 10 8 17 8 24 0 100 5 10
    240613125221_kkhg CCAAACAAACAGCATACATCAGCTGATATGAG(+AGGACA)GATCCTAACACATACACTAGAGGACTTCCAG NGG 10 8 17 8 24 0 100 5 10
    240613125220_tj09 AGTGTCAGGCTTTGGGACCTCCCTTTGAGCTG(+AGGACA)GATCCCATTTTGGGCCTGTTGCTGGACCTTC NGG 10 8 17 8 24 0 100 5 10
    240613125218_e0az GAAAAGAAGGTCCAGCAACAGGCCCAAAATGG(+AGGACA)GATCCAGCTCAAAGGGAGGTCCCAAAGCCTG NGG 10 8 17 8 24 0 100 5 10
    240613125216_ngy3 GACCAGCACGACTAGCACACATAGGGACCTGG(+AGGACA)GATCCTCTGTTTCCTCCTGTCTCTAGGAGAG NGG 10 8 17 8 24 0 100 5 10
    240613125215_9fng CCTTCCTCTCCTAGAGACAGGAGGAAACAGAG(+AGGACA)GATCCCAGGTCCCTATGTGTGCTAGTCGTGC NGG 10 8 17 8 24 0 100 5 10
    240613125214_v9qu TGAAGAAGTAGAGAAACAAGTCAGAGTGGTTG(+AGGACA)GATCCCCTGATTTACTCTGTCAGTACATTTT NGG 10 8 17 8 24 0 100 5 10
    240613125212_yl1d AAAAAAAAATGTACTGACAGAGTAAATCAGGG(+AGGACA)GATCCAACCACTCTGACTTGTTTCTCTACTT NGG 10 8 17 8 24 0 100 5 10