Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613125144_jqzp CTCTAGTGCTTTATAGCAAAAGCTATAAAGTG(+AGGACA)GTACCCGGGTGACCAGCATTGCCATGGTTAC NGG 10 8 17 8 24 0 100 5 10
    240613125141_ylt5 TGGATGTAACCATGGCAATGCTGGTCACCCGG(+AGGACA)GTACCACTTTATAGCTTTTGCTATAAAGCAC NGG 10 8 17 8 24 0 100 5 10
    240613125140_j3je CCACGCTGCTTCAAGCTAACTGCACCAGATTG(+AGGACA)GATCCAGCCATCAGCACTCCCAGAGACTCCT NGG 10 8 17 8 24 0 100 5 10
    240613125138_fhxv TTTTAAGGAGTCTCTGGGAGTGCTGATGGCTG(+AGGACA)GATCCAATCTGGTGCAGTTAGCTTGAAGCAG NGG 10 8 17 8 24 0 100 5 10
    240613125137_fyay TCACAAGAACATGATCTGTCCCGGCAGTGAAG(+AGGACA)GTACCAAGAGCAGCTTGGCCCATGGTGGCGA NGG 10 8 17 8 24 0 100 5 10
    240613125136_qcqs CCCATTCGCCACCATGGGCCAAGCTGCTCTTG(+AGGACA)GTACCTTCACTGCCGGGACAGATCATGTTCT NGG 10 8 17 8 24 0 100 5 10
    240613125134_6s8o CCTACAGTGACTTGATGTACCAGGGTGGGTTG(+AGGACA)GTACCCAGAGGAGCCTCCATCTTCTCAGAGG NGG 10 8 17 8 24 0 100 5 10
    240613125133_3q8o CTTCACCTCTGAGAAGATGGAGGCTCCTCTGG(+AGGACA)GTACCAACCCACCCTGGTACATCAAGTCACT NGG 10 8 17 8 24 0 100 5 10
    240613125131_72ha TTGTAAAAGGCCTACCTTCAAGACCACCCATG(+AGGACA)GATCCTCAAACCTATTAATCTGTTAAGGACG NGG 10 8 17 8 24 0 100 5 10
    240613125129_tpmp GGCTCCGTCCTTAACAGATTAATAGGTTTGAG(+AGGACA)GATCCATGGGTGGTCTTGAAGGTAGGCCTTT NGG 10 8 17 8 24 0 100 5 10