Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613125057_owss GGCATGGCTCCTCGCCGCAGCTGTCTCCCTTG(+AGGACA)GTACCGACCTCTCTGTGGGCTGGAGAGAGGC NGG 10 8 17 8 24 0 100 5 10
    240613125056_d0wo CTTAGGCCTCTCTCCAGCCCACAGAGAGGTCG(+AGGACA)GTACCAAGGGAGACAGCTGCGGCGAGGAGCC NGG 10 8 17 8 24 0 100 5 10
    240613125055_nf8v CCTTGTCAAACCAAGTTGTCATGGATGCTTTG(+AGGACA)GTACCCCTCCCCTCAGGGGCTTTGGTATTAC NGG 10 8 17 8 24 0 100 5 10
    240613125053_g2yq TTCTTGTAATACCAAAGCCCCTGAGGGGAGGG(+AGGACA)GTACCAAAGCATCCATGACAACTTGGTTTGA NGG 10 8 17 8 24 0 100 5 10
    240613125052_hxnp GTGGGGTTCCTGCGCTGTGTGTGCCTACTCAG(+AGGACA)GTACCCCAACATTGCTTTCCCTAGCTCTCTG NGG 10 8 17 8 24 0 100 5 10
    240613125050_dxr0 CACAGCAGAGAGCTAGGGAAAGCAATGTTGGG(+AGGACA)GTACCTGAGTAGGCACACACAGCGCAGGAAC NGG 10 8 17 8 24 0 100 5 10
    240613125049_0yt0 GCTCAATGCCATCATCACTGTGATTAAGTGAG(+AGGACA)GTACCAGGGTTCATAGCGTCGGAGACTGCTG NGG 10 8 17 8 24 0 100 5 10
    240613125047_p2ad AGCGTCAGCAGTCTCCGACGCTATGAACCCTG(+AGGACA)GTACCTCACTTAATCACAGTGATGATGGCAT NGG 10 8 17 8 24 0 100 5 10
    240613125045_t1ti ACAGGAACCAGTCCCTAAAAGTGCTCTTCTCG(+AGGACA)GTACCTGGCGAGGCAATCATTCCAGCGCTTC NGG 10 8 17 8 24 0 100 5 10
    240613125044_p7vs AAACAGAAGCGCTGGAATGATTGCCTCGCCAG(+AGGACA)GTACCGAGAAGAGCACTTTTAGGGACTGGTT NGG 10 8 17 8 24 0 100 5 10