Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613125043_ezn5 AGAATCTAGTAGCCACACAATGGGGGGCTCAG(+AGGACA)GTACCATTATTTACTACTTTCTTTTGACCTA NGG 10 8 17 8 24 0 100 5 10
    240613125041_joxr AGTCCTAGGTCAAAAGAAAGTAGTAAATAATG(+AGGACA)GTACCTGAGCCCCCCATTGTGTGGCTACTAG NGG 10 8 17 8 24 0 100 5 10
    240613125040_67d1 AAATACTCGATGTGCAATTACTTTGCACTTGG(+AGGACA)GATCCAGCAAGCCCCTCAGCGGTATCCTGAT NGG 10 8 17 8 24 0 100 5 10
    240613125039_x2xi TGGTTATCAGGATACCGCTGAGGGGCTTGCTG(+AGGACA)GATCCCAAGTGCAAAGTAATTGCACATCGAG NGG 10 8 17 8 24 0 100 5 10
    240613125037_c5qd AATGACATGAACTTATTAAGAATTAACTTAAG(+AGGACA)GATCCAATATATATATATATATATAATATGT NGG 10 8 17 8 24 0 100 5 10
    240613125033_s0kq ATATAACATATTATATATATATATATATATTG(+AGGACA)GATCCTTAAGTTAATTCTTAATAAGTTCATG NGG 10 8 17 8 24 0 100 5 10
    240613125032_te2e TGCTTAATTCTTTTAGCGAATGCACAGGTTAG(+AGGACA)GTACCACCGTGAGGAAAAGGAGGGTGTGGGG NGG 10 8 17 8 24 0 100 5 10
    240613125031_z52n TCATTCCCCACACCCTCCTTTTCCTCACGGTG(+AGGACA)GTACCTAACCTGTGCATTCGCTAAAAGAATT NGG 10 8 17 8 24 0 100 5 10
    240613125029_enh5 TGCCTTCATCTCAGAATTGCTCATCCCAGGTG(+AGGACA)GATCCCATGTGTCTGCAGTAGACCAGTCACC NGG 10 8 17 8 24 0 100 5 10
    240613125028_t8tg ACAAAGGTGACTGGTCTACTGCAGACACATGG(+AGGACA)GATCCACCTGGGATGAGCAATTCTGAGATGA NGG 10 8 17 8 24 0 100 5 10