Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613125324_ynn3 GGGTGGACCATGTAGAGACTGCCTTATCCAGG(+AGGACA)GATCCACCCCATAATCAGCATCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240613125322_pt98 GGTGTCAGCGTTTGGATGCTGATTATGGGGTG(+AGGACA)GATCCCTGGATAAGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240613125321_fwhh TCCCAAAGTTATTTAAACATCAGTAAGACAAG(+AGGACA)GTACCACTTCATACAGTGAAAAGTGGAATCT NGG 10 8 17 8 24 0 100 5 10
    240613125320_2gkv TCTGTAGATTCCACTTTTCACTGTATGAAGTG(+AGGACA)GTACCTTGTCTTACTGATGTTTAAATAACTT NGG 10 8 17 8 24 0 100 5 10
    240613125318_3r9e TCACTTCAATCTCTTCGTCACATATTGGGGTG(+AGGACA)GTACCAAGGAAGGTGACCAGTATTGTTTACA NGG 10 8 17 8 24 0 100 5 10
    240613125316_l9is TCATCTGTAAACAATACTGGTCACCTTCCTTG(+AGGACA)GTACCACCCCAATATGTGACGAAGAGATTGA NGG 10 8 17 8 24 0 100 5 10
    240613125315_7qlp CATGCTGGCAACATCTGATTAAAGGCAGTGAG(+AGGACA)GATCCAAGACGGATGGAGTGTCTTAGTGTGT NGG 10 8 17 8 24 0 100 5 10
    240613125314_rnxh GATGTACACACTAAGACACTCCATCCGTCTTG(+AGGACA)GATCCTCACTGCCTTTAATCAGATGTTGCCA NGG 10 8 17 8 24 0 100 5 10
    240613125313_8og6 AGTGATCGAGGGGGGAGGGCCCCTTGTGCATG(+AGGACA)GTACCATCTCTAGGCTGGTAGTCTTGAGTTC NGG 10 8 17 8 24 0 100 5 10
    240613125311_bth5 TTATAGAACTCAAGACTACCAGCCTAGAGATG(+AGGACA)GTACCATGCACAAGGGGCCCTCCCCCCTCGA NGG 10 8 17 8 24 0 100 5 10