Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613125426_36hb AAGGTTTGCCCTTTAGTTTAGAAACCAGATAG(+AGGACA)GATCCCATCCTTGAATTTTAAATGAAGTCAT NGG 10 8 17 8 24 0 100 5 10
    240613125424_ne7m TGCAAATGACTTCATTTAAAATTCAAGGATGG(+AGGACA)GATCCTATCTGGTTTCTAAACTAAAGGGCAA NGG 10 8 17 8 24 0 100 5 10
    240613125423_i6et GCAGTGCTGGTAGAATCCAGTAGAGGATATTG(+AGGACA)GATCCTCTGTGACTAGAATTACAGATAGTTA NGG 10 8 17 8 24 0 100 5 10
    240613125422_qsk0 GTTCATAACTATCTGTAATTCTAGTCACAGAG(+AGGACA)GATCCAATATCCTCTACTGGATTCTACCAGC NGG 10 8 17 8 24 0 100 5 10
    240613125421_92m8 CCAGACAAATGTAGTTTACTGCAGGCTTACTG(+AGGACA)GATCCTGACATTTAATTAAATATAACATAAT NGG 10 8 17 8 24 0 100 5 10
    240613125419_dpjw ACTAGATTATGTTATATTTAATTAAATGTCAG(+AGGACA)GATCCAGTAAGCCTGCAGTAAACTACATTTG NGG 10 8 17 8 24 0 100 5 10
    240613125418_9mbp ATAACATGGCAATAATGAAGTGCTGTTTCCTG(+AGGACA)GTACCTTGGGAATACTAAAGAAAGAAAAAGC NGG 10 8 17 8 24 0 100 5 10
    240613125415_1wnf GCAATGCTTTTTCTTTCTTTAGTATTCCCAAG(+AGGACA)GTACCAGGAAACAGCACTTCATTATTGCCAT NGG 10 8 17 8 24 0 100 5 10
    240613125414_y66c TTAAAAGAGAAACCAGAAACATCTAATCTGAG(+AGGACA)GATCCAGACAATAAATATTACATGGATCATT NGG 10 8 17 8 24 0 100 5 10
    240613125413_wooz CTCGGAATGATCCATGTAATATTTATTGTCTG(+AGGACA)GATCCTCAGATTAGATGTTTCTGGTTTCTCT NGG 10 8 17 8 24 0 100 5 10