Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613125520_26eb TAAGGCACATGGAAGACTGTTTATTTGCACAG(+AGGACA)GTACCGAAATTTTTTTAAGAACAGCTGTCAA NGG 10 8 17 8 24 0 100 5 10
    240613125519_s5re GAGATTTGACAGCTGTTCTTAAAAAAATTTCG(+AGGACA)GTACCTGTGCAAATAAACAGTCTTCCATGTG NGG 10 8 17 8 24 0 100 5 10
    240613125517_tmrz ATATCTGGGTTGGGACCTAGGATTTGTCCTCG(+AGGACA)GATCCTTTGGAACACAGAACATGGATTATGT NGG 10 8 17 8 24 0 100 5 10
    240613125515_2nqo GACGGACATAATCCATGTTCTGTGTTCCAAAG(+AGGACA)GATCCGAGGACAAATCCTAGGTCCCAACCCA NGG 10 8 17 8 24 0 100 5 10
    240613125514_7l8n GTCTAGCACTTACAGCATTAGCCTCCTTGATG(+AGGACA)GATCCACTACTACCTGCATTTTGACTTCTCA NGG 10 8 17 8 24 0 100 5 10
    240613125513_w8pm CTCTTTGAGAAGTCAAAATGCAGGTAGTAGTG(+AGGACA)GATCCATCAAGGAGGCTAATGCTGTAAGTGC NGG 10 8 17 8 24 0 100 5 10
    240613125511_0w16 TTGATGTGGGGGGGCCCAGCCCATTGTGGGTG(+AGGACA)GTACCATCCTGGGCTGGTGGTCCTGGGTTCT NGG 10 8 17 8 24 0 100 5 10
    240613125510_082k CTTATAGAACCCAGGACCACCAGCCCAGGATG(+AGGACA)GTACCACCCACAATGGGCTGGGCCCCCCCAC NGG 10 8 17 8 24 0 100 5 10
    240613125508_5s6a AAGAAAGAAATGCATTAGCCTAAGTCCTTGTG(+AGGACA)GATCCCAGGCTCCATTCTGCCATTCTCTTTC NGG 10 8 17 8 24 0 100 5 10
    240613125506_gf93 AGTAGGAAAGAGAATGGCAGAATGGAGCCTGG(+AGGACA)GATCCACAAGGACTTAGGCTAATGCATTTCT NGG 10 8 17 8 24 0 100 5 10