Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613125620_c3xl GTCATGTAACCCCTATGTCAGTGTCACCTTTG(+AGGACA)GTACCCCACCTCTTTGAGTCAATTCTGCTCC NGG 10 8 17 8 24 0 100 5 10
    240613125618_zhua GAAAAGGAGCAGAATTGACTCAAAGAGGTGGG(+AGGACA)GTACCAAAGGTGACACTGACATAGGGGTTAC NGG 10 8 17 8 24 0 100 5 10
    240613125617_5fzg TTGATTCTTTCAGGATGTGACGCCCACTCTGG(+AGGACA)GATCCAGTCCTGGTTGATGTCAGCATGCCAC NGG 10 8 17 8 24 0 100 5 10
    240613125615_j041 TGGAGGTGGCATGCTGACATCAACCAGGACTG(+AGGACA)GATCCCAGAGTGGGCGTCACATCCTGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240613125614_yga2 CCTGAGTATACCTGAGTCAGAGCGTGGAATCG(+AGGACA)GATCCAAGAAGTCAGTCGGCCTGGGGTCCAG NGG 10 8 17 8 24 0 100 5 10
    240613125613_z0l5 ATCGACTGGACCCCAGGCCGACTGACTTCTTG(+AGGACA)GATCCGATTCCACGCTCTGACTCAGGTATAC NGG 10 8 17 8 24 0 100 5 10
    240613125612_mtb0 CTATGACATCTGTGTGTCTGTGGTCTCACGAG(+AGGACA)GTACCTGGAGGTGTATGTGCACGGACATGGG NGG 10 8 17 8 24 0 100 5 10
    240613125610_cozt TACTTCCCATGTCCGTGCACATACACCTCCAG(+AGGACA)GTACCTCGTGAGACCACAGACACACAGATGT NGG 10 8 17 8 24 0 100 5 10
    240613125609_i9ax GAAAGGAAGGAAAGGGGTTTTGAGAAGAAAAG(+AGGACA)GATCCAAGCAATGGGGGCAGTTCTCACACCT NGG 10 8 17 8 24 0 100 5 10
    240613125607_lem9 CTCTGAGGTGTGAGAACTGCCCCCATTGCTTG(+AGGACA)GATCCTTTTCTTCTCAAAACCCCTTTCCTTC NGG 10 8 17 8 24 0 100 5 10