Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613125654_lcb2 CCCAGGAAATGCCTCCCTCCGGTCCAGGCAGG(+AGGACA)GATCCCAGCACACACACCCCTGCTGGTGGTG NGG 10 8 17 8 24 0 100 5 10
    240613125653_3k24 CCCTCCACCACCAGCAGGGGTGTGTGTGCTGG(+AGGACA)GATCCCTGCCTGGACCGGAGGGAGGCATTTC NGG 10 8 17 8 24 0 100 5 10
    240613125651_j1tg GTCCCACTTTCAATGGGAGCTATGAGGTATGG(+AGGACA)GTACCAGGCTTAGCAAGCAAGTTGTGGTCCC NGG 10 8 17 8 24 0 100 5 10
    240613125649_oepd CTGTGGGGACCACAACTTGCTTGCTAAGCCTG(+AGGACA)GTACCCATACCTCATAGCTCCCATTGAAAGT NGG 10 8 17 8 24 0 100 5 10
    240613125648_nw0k TTGTTCAGGGACTACTCAAGAAAATATCTTTG(+AGGACA)GATCCTTAGTTGGCCTTCAAGTCTATACATA NGG 10 8 17 8 24 0 100 5 10
    240613125647_99v2 TCTTATATGTATAGACTTGAAGGCCAACTAAG(+AGGACA)GATCCAAAGATATTTTCTTGAGTAGTCCCTG NGG 10 8 17 8 24 0 100 5 10
    240613125643_o2me ACACAGTTTGAAGCACTATGTTTCTTCCCCTG(+AGGACA)GTACCCTCTCTGTTCACAACTGCAGAAGCCT NGG 10 8 17 8 24 0 100 5 10
    240613125642_ozdb GAGAGAGGCTTCTGCAGTTGTGAACAGAGAGG(+AGGACA)GTACCAGGGGAAGAAACATAGTGCTTCAAAC NGG 10 8 17 8 24 0 100 5 10
    240613125640_1sm2 GTCATTGATGGGACAGGACAGGGAGATGGGAG(+AGGACA)GATCCCCCAAACACTAGGAAGCAAGCAGCAG NGG 10 8 17 8 24 0 100 5 10
    240613125638_mq5e TGTTTCTGCTGCTTGCTTCCTAGTGTTTGGGG(+AGGACA)GATCCTCCCATCTCCCTGTCCTGTCCCATCA NGG 10 8 17 8 24 0 100 5 10