Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613125755_e5ii CTGAGAACTTGAGACAAGGCTTACCACAGGAG(+AGGACA)GTACCCACTCCCCCATACCTCCTGGACAAGC NGG 10 8 17 8 24 0 100 5 10
    240613125753_ioxf TGTCTGCTTGTCCAGGAGGTATGGGGGAGTGG(+AGGACA)GTACCTCCTGTGGTAAGCCTTGTCTCAAGTT NGG 10 8 17 8 24 0 100 5 10
    240613125752_l7ve GAATCAGATAACAGAATCAGTGTTTCAGTCAG(+AGGACA)GATCCACACTGCCTGCCAGGCTGTGCTGTGG NGG 10 8 17 8 24 0 100 5 10
    240613125751_cg0i CACATCCACAGCACAGCCTGGCAGGCAGTGTG(+AGGACA)GATCCTGACTGAAACACTGATTCTGTTATCT NGG 10 8 17 8 24 0 100 5 10
    240613125749_b0r1 CGGTGAAGCCTTACGACTCCACCACAGTTCGG(+AGGACA)GATCCTAGATTCTGGCGTACATAGCTCTGCA NGG 10 8 17 8 24 0 100 5 10
    240613125748_g3x2 AGAGTTGCAGAGCTATGTACGCCAGAATCTAG(+AGGACA)GATCCCGAACTGTGGTGGAGTCGTAAGGCTT NGG 10 8 17 8 24 0 100 5 10
    240613125747_4nhs TGGTGCCATCCTAGGGACCAAGGTCTTTACAG(+AGGACA)GTACCTGGGCCTGTGGGAGACATTGTTTACA NGG 10 8 17 8 24 0 100 5 10
    240613125744_vjre TTGAGTGTAAACAATGTCTCCCACAGGCCCAG(+AGGACA)GTACCTGTAAAGACCTTGGTCCCTAGGATGG NGG 10 8 17 8 24 0 100 5 10
    240613125743_bkg5 CGGCGTCCTTCACTGATCGTTCTGTTTTTGAG(+AGGACA)GATCCCTGTGGCCCCGGGATCAGCCCATCAC NGG 10 8 17 8 24 0 100 5 10
    240613125741_8dxj AAGGAGTGATGGGCTGATCCCGGGGCCACAGG(+AGGACA)GATCCTCAAAAACAGAACGATCAGTGAAGGA NGG 10 8 17 8 24 0 100 5 10