Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613125740_l3ox CTAAGTAGCAGCCCTGACTTCTGGTCAGGTAG(+AGGACA)GATCCAACTGGCAGGAACCAGGCCAAGGAGT NGG 10 8 17 8 24 0 100 5 10
    240613125738_p2hk ATGTGACTCCTTGGCCTGGTTCCTGCCAGTTG(+AGGACA)GATCCTACCTGACCAGAAGTCAGGGCTGCTA NGG 10 8 17 8 24 0 100 5 10
    240613125737_9ou9 GCTGTCTTCAGACACACCAGAAGAGGGCATTG(+AGGACA)GATCCCATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240613125735_w6cr ACCACATGGTGGCTCACAACCATCTGTAATGG(+AGGACA)GATCCAATGCCCTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240613125734_6509 TTGATCCTGACTGTTCCTCAGAGAGGGCAACG(+AGGACA)GATCCCTGCTCAGTTTGCTGGCTCTTGTCAC NGG 10 8 17 8 24 0 100 5 10
    240613125732_ca9s GAGAAGTGACAAGAGCCAGCAAACTGAGCAGG(+AGGACA)GATCCGTTGCCCTCTCTGAGGAACAGTCAGG NGG 10 8 17 8 24 0 100 5 10
    240613125731_9icp GGAGGTTCAGTGACCAGTAGGCCTCCCCACTG(+AGGACA)GTACCACCACTTCCACTGTGAGACTGCAGAA NGG 10 8 17 8 24 0 100 5 10
    240613125729_mwmb TTGAGTTCTGCAGTCTCACAGTGGAAGTGGTG(+AGGACA)GTACCAGTGGGGAGGCCTACTGGTCACTGAA NGG 10 8 17 8 24 0 100 5 10
    240613125728_qy3x AAGATTCTAAAGAGCGTTGAAGTGTTTAAAAG(+AGGACA)GATCCACGAGGACAAGTTAGGTTGATGGTAT NGG 10 8 17 8 24 0 100 5 10
    240613125727_b0m2 GATACATACCATCAACCTAACTTGTCCTCGTG(+AGGACA)GATCCTTTTAAACACTTCAACGCTCTTTAGA NGG 10 8 17 8 24 0 100 5 10