Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613125725_0np6 GTGAGTGACTTGGGGGTGGGTTTGAGTCTGGG(+AGGACA)GTACCCAGTGACACAATTTTCTCATCTGGAA NGG 10 8 17 8 24 0 100 5 10
    240613125723_ijbr TAGAGTTCCAGATGAGAAAATTGTGTCACTGG(+AGGACA)GTACCCCAGACTCAAACCCACCCCCAAGTCA NGG 10 8 17 8 24 0 100 5 10
    240613125721_09mh GAAACGTCCTTCCTCCCCTTCGCCCTGCCTGG(+AGGACA)GATCCAGGTAGGGTGGTTGGACCTCTCCATC NGG 10 8 17 8 24 0 100 5 10
    240613125719_ls2a ACAAGGATGGAGAGGTCCAACCACCCTACCTG(+AGGACA)GATCCCAGGCAGGGCGAAGGGGAGGAAGGAC NGG 10 8 17 8 24 0 100 5 10
    240613125718_1cbt GAATGCCCTGGCATCTTGGTGGCATCTTGATG(+AGGACA)GATCCAGCTCAGTCAGGACAGCTTTGAGCAC NGG 10 8 17 8 24 0 100 5 10
    240613125717_5av7 GCTCTGTGCTCAAAGCTGTCCTGACTGAGCTG(+AGGACA)GATCCATCAAGATGCCACCAAGATGCCAGGG NGG 10 8 17 8 24 0 100 5 10
    240613125715_bm51 CTCCTTGCAGGAGGGGATACCACCATTTCAGG(+AGGACA)GATCCACAATTGAACCTCTGGCTTGATCCCT NGG 10 8 17 8 24 0 100 5 10
    240613125714_czai CAGGGAGGGATCAAGCCAGAGGTTCAATTGTG(+AGGACA)GATCCCTGAAATGGTGGTATCCCCTCCTGCA NGG 10 8 17 8 24 0 100 5 10
    240613125712_oami GCCATCTACTGAGCGAGTGTGAACTGGCCAGG(+AGGACA)GATCCACCAGGAAGAGGCATCATCTTCATTA NGG 10 8 17 8 24 0 100 5 10
    240613125710_vps8 CAATATAATGAAGATGATGCCTCTTCCTGGTG(+AGGACA)GATCCCTGGCCAGTTCACACTCGCTCAGTAG NGG 10 8 17 8 24 0 100 5 10