Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613125606_szw1 CTGACAGTGCCTTTCTGTCTTCAAAGACACTG(+AGGACA)GTACCAGCCAGGCAGTCAGGTGAACTCCTTC NGG 10 8 17 8 24 0 100 5 10
    240613125605_f31n GCTGGGAAGGAGTTCACCTGACTGCCTGGCTG(+AGGACA)GTACCAGTGTCTTTGAAGACAGAAAGGCACT NGG 10 8 17 8 24 0 100 5 10
    240613125603_qvq9 TATTGCTGTGTGTGCAGGACAGATGTTACCAG(+AGGACA)GTACCCTCAAATCATTCTCTACCTTATTTTT NGG 10 8 17 8 24 0 100 5 10
    240613125601_ogof GCTTCAAAAATAAGGTAGAGAATGATTTGAGG(+AGGACA)GTACCTGGTAACATCTGTCCTGCACACACAG NGG 10 8 17 8 24 0 100 5 10
    240613125600_tooj AGCACCTTCCTGGAGCCCCGTGCCCTTGCTGG(+AGGACA)GATCCTGAGACAACTGTCAGCTGAGCCCTGT NGG 10 8 17 8 24 0 100 5 10
    240613125559_bpe5 TTCCCACAGGGCTCAGCTGACAGTTGTCTCAG(+AGGACA)GATCCCAGCAAGGGCACGGGGCTCCAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240613125557_d82u ACAGACCAGGCAGGGCTGGCTGGAGTTGAGGG(+AGGACA)GATCCCGGTCCAAGAGAGAGAGAGCGCTGGG NGG 10 8 17 8 24 0 100 5 10
    240613125554_qfw5 CAGCGCCCAGCGCTCTCTCTCTCTTGGACCGG(+AGGACA)GATCCCCTCAACTCCAGCCAGCCCTGCCTGG NGG 10 8 17 8 24 0 100 5 10
    240613125553_nfyc CGAGAGGGCGTCAAAGAGGAAGCCTGGGCTGG(+AGGACA)GATCCTTAGGCTCAGCTGTCTGTCTAATCCC NGG 10 8 17 8 24 0 100 5 10
    240613125551_en1a GCTCTGGGATTAGACAGACAGCTGAGCCTAAG(+AGGACA)GATCCCAGCCCAGGCTTCCTCTTTGACGCCC NGG 10 8 17 8 24 0 100 5 10