Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613125357_b6oy ACGAAATGGACAAATTTCTGGACAGATACCAG(+AGGACA)GTACCAAAGTTGAATCAGGATCAAGTTGACC NGG 10 8 17 8 24 0 100 5 10
    240613125356_dwn9 TAGAAGGTCAACTTGATCCTGATTCAACTTTG(+AGGACA)GTACCTGGTATCTGTCCAGAAATTTGTCCAT NGG 10 8 17 8 24 0 100 5 10
    240613125355_0zfi ATCTGTGTAAAGTTTGCATTAGTTAGAAGATG(+AGGACA)GTACCGAGTTTAATTGACATAATAAAAGCTT NGG 10 8 17 8 24 0 100 5 10
    240613125354_1zio TGGGCAAGCTTTTATTATGTCAATTAAACTCG(+AGGACA)GTACCATCTTCTAACTAATGCAAACTTTACA NGG 10 8 17 8 24 0 100 5 10
    240613125352_xex5 GGTGTCAGCGTTTGGATGCTGATTATGGGGTG(+AGGACA)GATCCCTGGATAAGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240613125349_at2i GGATGGACCATGTAGAGACTGCCTTATCCAGG(+AGGACA)GATCCACCCCATAATCAGCATCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240613125348_3j7f TTCTGGATATATGCCCAGGAGAGGTATTGCTG(+AGGACA)GATCCTCCGGTAGTACTATGTCCAATTTACT NGG 10 8 17 8 24 0 100 5 10
    240613125346_ttfk TCCTCAGTAAATTGGACATAGTACTACCGGAG(+AGGACA)GATCCAGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240613125344_ibr5 TTCTGGATATATGCCCAGGAGAGGTATTGTGG(+AGGACA)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240613125342_fs42 TCCTCAGAAAATTGGACATAGTACTACCGGAG(+AGGACA)GATCCCACAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10