Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613125310_husy AGCCCTTGGAGGAGTCTAGAAGATGGTGAGTG(+AGGACA)GATCCCAGACATTGGACCGGTGGAGTTTGAT NGG 10 8 17 8 24 0 100 5 10
    240613125309_873v GCAAAATCAAACTCCACCGGTCCAATGTCTGG(+AGGACA)GATCCACTCACCATCTTCTAGACTCCTCCAA NGG 10 8 17 8 24 0 100 5 10
    240613125307_4l7b AAAAAATGAGACTTTGACAATTAGTTGGAAAG(+AGGACA)GATCCAACTGAAATTGTGGTACATTTTACTT NGG 10 8 17 8 24 0 100 5 10
    240613125306_irau TGTGTAAGTAAAATGTACCACAATTTCAGTTG(+AGGACA)GATCCTTTCCAACTAATTGTCAAAGTCTCAT NGG 10 8 17 8 24 0 100 5 10
    240613125305_zfev TGAGTGTCTGTTCTGTCCTGTTGGACCTCATG(+AGGACA)GTACCCACACTTGAGGCAGCTTGTTTCCTTA NGG 10 8 17 8 24 0 100 5 10
    240613125304_kwxt CTGGGTAAGGAAACAAGCTGCCTCAAGTGTGG(+AGGACA)GTACCATGAGGTCCAACAGGACAGAACAGAC NGG 10 8 17 8 24 0 100 5 10
    240613125302_nfvc TTGTTAAAGGATTTGAGGGTTTTTCTCTGCAG(+AGGACA)GTACCACCTGTAAATAAGAAGAAAGTAGGGG NGG 10 8 17 8 24 0 100 5 10
    240613125301_v30b TTTGGCCCCTACTTTCTTCTTATTTACAGGTG(+AGGACA)GTACCTGCAGAGAAAAACCCTCAAATCCTTT NGG 10 8 17 8 24 0 100 5 10
    240613125259_uwn2 ATTTCTTCAAAGAGAGACAAGGTTCATGCCTG(+AGGACA)GTACCATTTTTTTTCTAATGAAATTAACTTT NGG 10 8 17 8 24 0 100 5 10
    240613125257_h7hn CTAAAAAAGTTAATTTCATTAGAAAAAAAATG(+AGGACA)GTACCAGGCATGAACCTTGTCTCTCTTTGAA NGG 10 8 17 8 24 0 100 5 10