Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613124247_gbnv GATTGAGCTCCAGCTATTCAGCTTGGCAGCAG(+AGGACA)GTACCTTGAGCCCGAGCCACCTTGCTAGCAC NGG 10 8 17 8 24 0 100 5 10
    240613124246_nwax GACTTGTGCTAGCAAGGTGGCTCGGGCTCAAG(+AGGACA)GTACCTGCTGCCAAGCTGAATAGCTGGAGCT NGG 10 8 17 8 24 0 100 5 10
    240613124244_0tk9 TTTTGAAAGGTAAGTGTGACCAGAGGTGGCTG(+AGGACA)GATCCAGATTTAAAATACACAACCATTAGCT NGG 10 8 17 8 24 0 100 5 10
    240613124243_qng8 AACTCAGCTAATGGTTGTGTATTTTAAATCTG(+AGGACA)GATCCAGCCACCTCTGGTCACACTTACCTTT NGG 10 8 17 8 24 0 100 5 10
    240613124242_1iax AAAAGAATATACCTTCTTCTCAGCACCTCATG(+AGGACA)GTACCTTTTCCAAAACTGACCATGTAACTGG NGG 10 8 17 8 24 0 100 5 10
    240613124241_eju3 TGTGACCAGTTACATGGTCAGTTTTGGAAAAG(+AGGACA)GTACCATGAGGTGCTGAGAAGAAGGTATATT NGG 10 8 17 8 24 0 100 5 10
    240613124240_nfce AAGAAAATATGAGGCCAAGAATCTGGGGACAG(+AGGACA)GATCCTTTTAGTCTCCATCTGAGCCTCAGAG NGG 10 8 17 8 24 0 100 5 10
    240613124238_d4l9 GTTAACTCTGAGGCTCAGATGGAGACTAAAAG(+AGGACA)GATCCTGTCCCCAGATTCTTGGCCTCATATT NGG 10 8 17 8 24 0 100 5 10
    240613124236_f1u0 GGATGGACCATGTAGAGACTGTCATATCCAGG(+AGGACA)GATCCACCCCATAATCAGCATCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240613124234_tcu4 GGTGTCAGCGTTTGGATGCTGATTATGGGGTG(+AGGACA)GATCCCTGGATATGACAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10