Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613124318_u3sg GCAGCAGACTGGGACCCTGGGAGGGCTGGCAG(+AGGACA)GATCCTGCTAGGGCCCTGCTTTACCATCTCT NGG 10 8 17 8 24 0 100 5 10
    240613124316_2ox3 CTGAAAGAGATGGTAAAGCAGGGCCCTAGCAG(+AGGACA)GATCCTGCCAGCCCTCCCAGGGTCCCAGTCT NGG 10 8 17 8 24 0 100 5 10
    240613124315_b3as AAAAGGATATACCTTCTTCTCAGCACCTCATG(+AGGACA)GTACCTTCTCCAAAATTGACCACATAATAGG NGG 10 8 17 8 24 0 100 5 10
    240613124314_n9rk TGTGACCTATTATGTGGTCAATTTTGGAGAAG(+AGGACA)GTACCATGAGGTGCTGAGAAGAAGGTATATC NGG 10 8 17 8 24 0 100 5 10
    240613124312_zo0g ACGAAATGGACAAATTTCTGGACAGATACCAG(+AGGACA)GTACCAAAGTTGAATCAGGATCAAGTTGACC NGG 10 8 17 8 24 0 100 5 10
    240613124311_q4vw TAGAAGGTCAACTTGATCCTGATTCAACTTTG(+AGGACA)GTACCTGGTATCTGTCCAGAAATTTGTCCAT NGG 10 8 17 8 24 0 100 5 10
    240613124310_peuu CATTAGGTAATCATTCCTGCCTTTAAAACAAG(+AGGACA)GATCCTGCTGGCTCCTCTTTTCAGAGTCACT NGG 10 8 17 8 24 0 100 5 10
    240613124309_hegs CTTACAGTGACTCTGAAAAGAGGAGCCAGCAG(+AGGACA)GATCCTTGTTTTAAAGGCAGGAATGATTACC NGG 10 8 17 8 24 0 100 5 10
    240613124307_4vp2 GGTGTCAGCGTTTGGAAGCTGATTATGGGATG(+AGGACA)GATCCCTGGATATGGCAGTCTCTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240613124305_bd6b GGATGGACCATCTAGAGACTGCCATATCCAGG(+AGGACA)GATCCATCCCATAATCAGCTTCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10