Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613124408_kswf TTAAGGCAAGTGTGTCATGGTGCTGAGCGTGG(+AGGACA)GTACCAATTAGATGTTTCAGTGACACATGCT NGG 10 8 17 8 24 0 100 5 10
    240613124406_58ar CACAAAGCATGTGTCACTGAAACATCTAATTG(+AGGACA)GTACCCACGCTCAGCACCATGACACACTTGC NGG 10 8 17 8 24 0 100 5 10
    240613124405_0m23 CTTGATCCTGATCCACAGTTAATATTGTTATG(+AGGACA)GATCCTGGTCTACAAAGTGAGTTCCAGGACA NGG 10 8 17 8 24 0 100 5 10
    240613124403_y2gm CTGGCTGTCCTGGAACTCACTTTGTAGACCAG(+AGGACA)GATCCATAACAATATTAACTGTGGATCAGGA NGG 10 8 17 8 24 0 100 5 10
    240613124402_rf4s ATGATGTTACTGCTGCATCCAGCTTTTACATG(+AGGACA)GATCCTAATTGAGGTCCTCATATTTGCATAG NGG 10 8 17 8 24 0 100 5 10
    240613124400_knm8 GCCTCCTATGCAAATATGAGGACCTCAATTAG(+AGGACA)GATCCATGTAAAAGCTGGATGCAGCAGTAAC NGG 10 8 17 8 24 0 100 5 10
    240613124358_nt82 GTTCTCGGGGTGGTTCCTCTGTCAGCTGCCAG(+AGGACA)GATCCTGTGCTAGAGACAGCCTGCCAATGAC NGG 10 8 17 8 24 0 100 5 10
    240613124357_xl2a TCTCTGTCATTGGCAGGCTGTCTCTAGCACAG(+AGGACA)GATCCTGGCAGCTGACAGAGGAACCACCCCG NGG 10 8 17 8 24 0 100 5 10
    240613124355_4mr8 CAGATGATGGCTCTGGTGGTCATGTTAGGAGG(+AGGACA)GATCCTGAGGTCCCTGCTCTTCACCATGCAG NGG 10 8 17 8 24 0 100 5 10
    240613124353_buss AGTCTCTGCATGGTGAAGAGCAGGGACCTCAG(+AGGACA)GATCCCTCCTAACATGACCACCAGAGCCATC NGG 10 8 17 8 24 0 100 5 10