Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613124422_6tlf TGGCTACTTTACGTGTACGTGAATGAGGCTGG(+AGGACA)GTACCAGATGTAAAGGCTAAGGGAGAGGAGA NGG 10 8 17 8 24 0 100 5 10
    240613124421_vdbp TGTAGTCTCCTCTCCCTTAGCCTTTACATCTG(+AGGACA)GTACCCAGCCTCATTCACGTACACGTAAAGT NGG 10 8 17 8 24 0 100 5 10
    240613124419_706e TTAACTGGAGTTTAATTAATAAACTATTTAGG(+AGGACA)GATCCACCACCAGTCATATAGACATATTAAA NGG 10 8 17 8 24 0 100 5 10
    240613124418_hjfb TTTGTTTTAATATGTCTATATGACTGGTGGTG(+AGGACA)GATCCCTAAATAGTTTATTAATTAAACTCCA NGG 10 8 17 8 24 0 100 5 10
    240613124417_hrww CATGTAGTGGCCTGTGGAGGTCATAAACAGTG(+AGGACA)GATCCCTTGGAGCTGGAGTCACAGTTGGCTA NGG 10 8 17 8 24 0 100 5 10
    240613124414_dzwo TCTCGTAGCCAACTGTGACTCCAGCTCCAAGG(+AGGACA)GATCCACTGTTTATGACCTCCACAGGCCACT NGG 10 8 17 8 24 0 100 5 10
    240613124413_xuac CCAAGTTCCTCAGCACTCTCTGAATGACTGTG(+AGGACA)GATCCTTGAGGAGGAGGAGGAGCAACGCGAA NGG 10 8 17 8 24 0 100 5 10
    240613124412_zso7 CCGGGTTCGCGTTGCTCCTCCTCCTCCTCAAG(+AGGACA)GATCCACAGTCATTCAGAGAGTGCTGAGGAA NGG 10 8 17 8 24 0 100 5 10
    240613124410_unn8 ATTCCTGAAAGAAAAGCCAAAATCACAAGCAG(+AGGACA)GATCCTTTAAAGTCTTTGTGCTCCCAGCGTT NGG 10 8 17 8 24 0 100 5 10
    240613124409_cvd6 GGTAAAACGCTGGGAGCACAAAGACTTTAAAG(+AGGACA)GATCCTGCTTGTGATTTTGGCTTTTCTTTCA NGG 10 8 17 8 24 0 100 5 10