Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613124334_vszi TTCATACATTTAGAAGGCATATAAAGAGATAG(+AGGACA)GATCCCTTTAGCCCAGGCTGATCTTGAACTT NGG 10 8 17 8 24 0 100 5 10
    240613124332_xs9i ACTACAAGTTCAAGATCAGCCTGGGCTAAAGG(+AGGACA)GATCCTATCTCTTTATATGCCTTCTAAATGT NGG 10 8 17 8 24 0 100 5 10
    240613124330_ttyi AGTGATTAAGAGCCCTGGTTGCTCTTCCAAAG(+AGGACA)GATCCAGGCTCGATTCCCAGAACTCACATGG NGG 10 8 17 8 24 0 100 5 10
    240613124328_kum8 AGCCACCATGTGAGTTCTGGGAATCGAGCCTG(+AGGACA)GATCCTTTGGAAGAGCAACCAGGGCTCTTAA NGG 10 8 17 8 24 0 100 5 10
    240613124327_nk74 TGCTCATTAACTGATGTCTCTCCAAAAAGCTG(+AGGACA)GTACCTCACCTTCAGCTTTGGGCAGAGTCGT NGG 10 8 17 8 24 0 100 5 10
    240613124326_61n3 TCTGCACGACTCTGCCCAAAGCTGAAGGTGAG(+AGGACA)GTACCAGCTTTTTGGAGAGACATCAGTTAAT NGG 10 8 17 8 24 0 100 5 10
    240613124324_vg1w GCCAGCATGCAAGGCTCTGGGGTCAGTATCTG(+AGGACA)GTACCAGAGGTGGGGTGGTAGGGTATAAACT NGG 10 8 17 8 24 0 100 5 10
    240613124323_v12h CAGTTAGTTTATACCCTACCACCCCACCTCTG(+AGGACA)GTACCAGATACTGACCCCAGAGCCTTGCATG NGG 10 8 17 8 24 0 100 5 10
    240613124321_4x4m TATTTGTAAAGACTGGGACCCTACCTGAAGGG(+AGGACA)GATCCAACCACTCCACAGGAAGGGCTAAAAC NGG 10 8 17 8 24 0 100 5 10
    240613124319_djr0 AAAATGTTTTAGCCCTTCCTGTGGAGTGGTTG(+AGGACA)GATCCCCTTCAGGTAGGGTCCCAGTCTTTAC NGG 10 8 17 8 24 0 100 5 10