Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613124450_fukb GGGAAACTATTACTTCAAATGAACAGTATTGG(+AGGACA)GATCCTTCTGCCTTACTTAGTTACCTAGTAT NGG 10 8 17 8 24 0 100 5 10
    240613124449_66p2 TTCAGATACTAGGTAACTAAGTAAGGCAGAAG(+AGGACA)GATCCCAATACTGTTCATTTGAAGTAATAGT NGG 10 8 17 8 24 0 100 5 10
    240613124447_r2ky TCCTGGCAGAGCTCCACACGGCCTTCCTGGTG(+AGGACA)GATCCTAAAGGAGGGCGCATAGCAAGGTGCT NGG 10 8 17 8 24 0 100 5 10
    240613124446_ojki TCCGGAGCACCTTGCTATGCGCCCTCCTTTAG(+AGGACA)GATCCACCAGGAAGGCCGTGTGGAGCTCTGC NGG 10 8 17 8 24 0 100 5 10
    240613124445_x0ab ATGTCACAGGGCTGTGTCCACACCAGCAGCAG(+AGGACA)GATCCTTGGGTACCCTTTGCTGCTTCCTTTC NGG 10 8 17 8 24 0 100 5 10
    240613124442_eo92 GAAGGGAAAGGAAGCAGCAAAGGGTACCCAAG(+AGGACA)GATCCTGCTGCTGGTGTGGACACAGCCCTGT NGG 10 8 17 8 24 0 100 5 10
    240613124441_yt21 TCTTCTGAGATAATAAGCACAATCCTGAAATG(+AGGACA)GATCCAATAATTCAGCTAGATATTATAGATT NGG 10 8 17 8 24 0 100 5 10
    240613124440_o5e2 ATACTAATCTATAATATCTAGCTGAATTATTG(+AGGACA)GATCCATTTCAGGATTGTGCTTATTATCTCA NGG 10 8 17 8 24 0 100 5 10
    240613124439_duy2 ACTTCTTTTCTTCCTCTCCTCTAAGTGAACAG(+AGGACA)GATCCCCTTTTTGGTCCATTTGTCCATGCCT NGG 10 8 17 8 24 0 100 5 10
    240613124437_32q8 GTGAGAGGCATGGACAAATGGACCAAAAAGGG(+AGGACA)GATCCTGTTCACTTAGAGGAGAGGAAGAAAA NGG 10 8 17 8 24 0 100 5 10