Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613124553_ogbx TGAGTTTGCCAGGAGCCCTGGGGTGCAGGCAG(+AGGACA)GATCCCTGCAGCCCCACAGCCAACTTTTCCT NGG 10 8 17 8 24 0 100 5 10
    240613124551_7cot GAAGAAGGAAAAGTTGGCTGTGGGGCTGCAGG(+AGGACA)GATCCTGCCTGCACCCCAGGGCTCCTGGCAA NGG 10 8 17 8 24 0 100 5 10
    240613124550_18id AGCCGTGGAACACGTCTCTTAGGGTGTGCTTG(+AGGACA)GATCCAGAGCCCTGCAGGTCTCTGGGGTTTG NGG 10 8 17 8 24 0 100 5 10
    240613124549_4kyl AGTGACAAACCCCAGAGACCTGCAGGGCTCTG(+AGGACA)GATCCAAGCACACCCTAAGAGACGTGTTCCA NGG 10 8 17 8 24 0 100 5 10
    240613124547_f6sj TCTCTCTATCCCTTAAAAAGAACTATCTCCAG(+AGGACA)GTACCCAGTCCTGTTGTAGAAACCACCTGAT NGG 10 8 17 8 24 0 100 5 10
    240613124545_jova TGGGCATCAGGTGGTTTCTACAACAGGACTGG(+AGGACA)GTACCTGGAGATAGTTCTTTTTAAGGGATAG NGG 10 8 17 8 24 0 100 5 10
    240613124544_qcce TTGTTCTCCAGACCTGACCCAGGTAGGCAAGG(+AGGACA)GTACCCTCCCAGTCTCACCTACCATACTGTG NGG 10 8 17 8 24 0 100 5 10
    240613124542_mw19 GATGGCACAGTATGGTAGGTGAGACTGGGAGG(+AGGACA)GTACCCTTGCCTACCTGGGTCAGGTCTGGAG NGG 10 8 17 8 24 0 100 5 10
    240613124541_jsfs TGCTAAACCGTTAAGTAGCTGGAAGATATCTG(+AGGACA)GATCCTTCTATGCCCTCTCACCCGTAGGCCC NGG 10 8 17 8 24 0 100 5 10
    240613124539_cllv GAAGGGGGCCTACGGGTGAGAGGGCATAGAAG(+AGGACA)GATCCAGATATCTTCCAGCTACTTAACGGTT NGG 10 8 17 8 24 0 100 5 10