Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613124537_p7ep TTACAGTGACCCCCTTTGTTTGCAGTGAAGGG(+AGGACA)GATCCTTTGCTTCTCGGTCCTCAACACGATG NGG 10 8 17 8 24 0 100 5 10
    240613124536_u7r9 ACCCTCATCGTGTTGAGGACCGAGAAGCAAAG(+AGGACA)GATCCCCTTCACTGCAAACAAAGGGGGTCAC NGG 10 8 17 8 24 0 100 5 10
    240613124535_z84q GCAAAAGTGTCTTCCCCCTCCCCCGACTTCAG(+AGGACA)GATCCTGAAGGAAGGACTGGGTGCGGTGCGG NGG 10 8 17 8 24 0 100 5 10
    240613124533_dne5 TCTTGCCGCACCGCACCCAGTCCTTCCTTCAG(+AGGACA)GATCCTGAAGTCGGGGGAGGGGGAAGACACT NGG 10 8 17 8 24 0 100 5 10
    240613124532_ysoq AGCTGGTGCCCCCTCCCCAAAGACAACTGTAG(+AGGACA)GTACCATCTAGTGAACAGCAAGACCTAGACT NGG 10 8 17 8 24 0 100 5 10
    240613124531_dj6g ATTCCAGTCTAGGTCTTGCTGTTCACTAGATG(+AGGACA)GTACCTACAGTTGTCTTTGGGGAGGGGGCAC NGG 10 8 17 8 24 0 100 5 10
    240613124530_sq4u GCTAGGCAAGACAGAATGGAGGGCATATTTAG(+AGGACA)GATCCCACCCAGCAGTCTACAGCTCATCTCC NGG 10 8 17 8 24 0 100 5 10
    240613124528_l4o2 TCTTTGGAGATGAGCTGTAGACTGCTGGGTGG(+AGGACA)GATCCTAAATATGCCCTCCATTCTGTCTTGC NGG 10 8 17 8 24 0 100 5 10
    240613124526_s0hq AGTAGACTAGGTTAGCTGAGGAGTGTGCAAGG(+AGGACA)GATCCCTTACCTCTCCTTCCCTGCTTTCTGC NGG 10 8 17 8 24 0 100 5 10
    240613124525_vizq AGAGAGCAGAAAGCAGGGAAGGAGAGGTAAGG(+AGGACA)GATCCCTTGCACACTCCTCAGCTAACCTAGT NGG 10 8 17 8 24 0 100 5 10