Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613124522_g7lw AGCTAAGTCTATAGATCCAACTTTCTGACCTG(+AGGACA)GATCCAGACAACACTACTTTGGAAAGAGTGT NGG 10 8 17 8 24 0 100 5 10
    240613124521_6ntx TATTAACACTCTTTCCAAAGTAGTGTTGTCTG(+AGGACA)GATCCAGGTCAGAAAGTTGGATCTATAGACT NGG 10 8 17 8 24 0 100 5 10
    240613124520_m9bu AAGGAGTTTTACTCCTTGGTTTCAAAGCAGAG(+AGGACA)GATCCGCCTATGGACAGGTTCATGAAAATGA NGG 10 8 17 8 24 0 100 5 10
    240613124518_u7e7 ATCCATCATTTTCATGAACCTGTCCATAGGCG(+AGGACA)GATCCTCTGCTTTGAAACCAAGGAGTAAAAC NGG 10 8 17 8 24 0 100 5 10
    240613124516_4tvb GGTATCATCTCCAGTTTCAGGCATCCAGTGGG(+AGGACA)GATCCCTGACAGGTGGGAGAGAACTACTATA NGG 10 8 17 8 24 0 100 5 10
    240613124514_mogb ATTATTATAGTAGTTCTCTCCCACCTGTCAGG(+AGGACA)GATCCCCACTGGATGCCTGAAACTGGAGATG NGG 10 8 17 8 24 0 100 5 10
    240613124513_zdcu AAAGGATAACTTTTCTTTCTTTTGACTATGTG(+AGGACA)GATCCTACATATTGAACTCAGATAGTCAGGG NGG 10 8 17 8 24 0 100 5 10
    240613124512_kyal GCTAACCCTGACTATCTGAGTTCAATATGTAG(+AGGACA)GATCCACATAGTCAAAAGAAAGAAAAGTTAT NGG 10 8 17 8 24 0 100 5 10
    240613124511_fjki GGGGGGGGGCGGGGGTGGTGTGGTCTGAACAG(+AGGACA)GATCCCACAGTAGCTCAGGCTGGCCTTGAAC NGG 10 8 17 8 24 0 100 5 10
    240613124509_p8yh AGAAAGTTCAAGGCCAGCCTGAGCTACTGTGG(+AGGACA)GATCCTGTTCAGACCACACCACCCCCGCCCC NGG 10 8 17 8 24 0 100 5 10