Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613124637_3v24 GGACTGATCCGAAGACTCTATGCTGGAACCTG(+AGGACA)GATCCTCATCATCTATGTATATAAGATCTTT NGG 10 8 17 8 24 0 100 5 10
    240613124635_lapf TGCCAAAAGATCTTATATACATAGATGATGAG(+AGGACA)GATCCAGGTTCCAGCATAGAGTCTTCGGATC NGG 10 8 17 8 24 0 100 5 10
    240613124634_sion CTGTAAGACGTTTGCTTTGTAACTTGGTCACG(+AGGACA)GATCCAAGCCCATGCTGGGGGAAATGGCACT NGG 10 8 17 8 24 0 100 5 10
    240613124633_7tix GCACTAGTGCCATTTCCCCCAGCATGGGCTTG(+AGGACA)GATCCGTGACCAAGTTACAAAGCAAACGTCT NGG 10 8 17 8 24 0 100 5 10
    240613124631_f2ay CTCAGTCTGCTCTCCACTGTCCATGTTGTTGG(+AGGACA)GATCCGGCACAACCGAGCCTGTGTACTGGGA NGG 10 8 17 8 24 0 100 5 10
    240613124630_qsjy GAGGGTCCCAGTACACAGGCTCGGTTGTGCCG(+AGGACA)GATCCCAACAACATGGACAGTGGAGAGCAGA NGG 10 8 17 8 24 0 100 5 10
    240613124628_7x85 GTAAATATGCAAAAAGCGTTTCCTAATGGCTG(+AGGACA)GATCCATGGTGTCTGGCCCAATGAAGTAAGA NGG 10 8 17 8 24 0 100 5 10
    240613124626_ymgq CTTTCTCTTACTTCATTGGGCCAGACACCATG(+AGGACA)GATCCAGCCATTAGGAAACGCTTTTTGCATA NGG 10 8 17 8 24 0 100 5 10
    240613124625_z9k0 AGACAGCTTTCCGGGTCCAGATATCTAATCTG(+AGGACA)GATCCATGCTAACAGTACAGCACACAAGCTG NGG 10 8 17 8 24 0 100 5 10
    240613124624_n5d6 AGCTACAGCTTGTGTGCTGTACTGTTAGCATG(+AGGACA)GATCCAGATTAGATATCTGGACCCGGAAAGC NGG 10 8 17 8 24 0 100 5 10