Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613123855_xt2g AGTCAACAGGGACAGCAGAATGACCACTAATG(+AGGACA)GATCCTTAATCTTCTTCTAGTAGCTTTGGGG NGG 10 8 17 8 24 0 100 5 10
    240613123854_h0uu ACTTCCCCCAAAGCTACTAGAAGAAGATTAAG(+AGGACA)GATCCATTAGTGGTCATTCTGCTGTCCCTGT NGG 10 8 17 8 24 0 100 5 10
    240613123852_gxwc GATGCAGAGATACGTCTGGGTGAGCTAACCAG(+AGGACA)GATCCTCATTACCTAGGTCATCAAGGGTCGA NGG 10 8 17 8 24 0 100 5 10
    240613123851_47hz TACAATCGACCCTTGATGACCTAGGTAATGAG(+AGGACA)GATCCTGGTTAGCTCACCCAGACGTATCTCT NGG 10 8 17 8 24 0 100 5 10
    240613123850_tjto CTTCTTTTTTTTTTTTTTTTTTAGCAAAAGTG(+AGGACA)GTACCAGTGCAGGGCCAAGTGCGTTGCTCTC NGG 10 8 17 8 24 0 100 5 10
    240613123849_283q CAGAAGAGAGCAACGCACTTGGCCCTGCACTG(+AGGACA)GTACCACTTTTGCTAAAAAAAAAAAAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240613123848_q1p9 AAGCAGACCACTTCAGGTTCTTCCAGCTTGCG(+AGGACA)GTACCATGGCTTTGAGGGTCCTGCTGCTCGG NGG 10 8 17 8 24 0 100 5 10
    240613123846_kpv0 TGGGGCCGAGCAGCAGGACCCTCAAAGCCATG(+AGGACA)GTACCGCAAGCTGGAAGAACCTGAAGTGGTC NGG 10 8 17 8 24 0 100 5 10
    240613123843_kakt ATGTCCCCAAAGCCATGGCAGGGCCTCCCTTG(+AGGACA)GATCCTTTCATCAGGGGAAGGAGTGAGGTAT NGG 10 8 17 8 24 0 100 5 10
    240613123842_y4f0 CCCTTATACCTCACTCCTTCCCCTGATGAAAG(+AGGACA)GATCCAAGGGAGGCCCTGCCATGGCTTTGGG NGG 10 8 17 8 24 0 100 5 10