Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613123948_640n GTTCTGTAGATGAGGCTGGACTTAAACTTAGG(+AGGACA)GATCCACCTGTCTCTGCTCAAGTGATGGCAT NGG 10 8 17 8 24 0 100 5 10
    240613123947_igom TAATGGATAAGGCTTTATTTCTCTGCTGGAAG(+AGGACA)GATCCATCTGGGAGAAAGAGAAGGGCAGGGG NGG 10 8 17 8 24 0 100 5 10
    240613123946_2ou1 TATGGCCCCTGCCCTTCTCTTTCTCCCAGATG(+AGGACA)GATCCTTCCAGCAGAGAAATAAAGCCTTATC NGG 10 8 17 8 24 0 100 5 10
    240613123944_o4aq TCACAAAGGGGACCCCCTCCCAGGGCCTACAG(+AGGACA)GATCCAGGGATTGAATGCTCAGGGAAGCACT NGG 10 8 17 8 24 0 100 5 10
    240613123942_0ch5 GTGGTAGTGCTTCCCTGAGCATTCAATCCCTG(+AGGACA)GATCCTGTAGGCCCTGGGAGGGGGTCCCCTT NGG 10 8 17 8 24 0 100 5 10
    240613123940_jp7w ACTACCAAATTGCTTGGCCCTTACCCTTCCAG(+AGGACA)GATCCCTGCATATCCTCTCCAGCCCCAACCC NGG 10 8 17 8 24 0 100 5 10
    240613123938_jntk GGGGTGGGTTGGGGCTGGAGAGGATATGCAGG(+AGGACA)GATCCTGGAAGGGTAAGGGCCAAGCAATTTG NGG 10 8 17 8 24 0 100 5 10
    240613123937_71qs TTGCTACTGGGCCACAGAATGGGTAACACGTG(+AGGACA)GTACCAACTTTGATTTGGTCAAAGTTGATGT NGG 10 8 17 8 24 0 100 5 10
    240613123936_i1g5 AGGCAACATCAACTTTGACCAAATCAAAGTTG(+AGGACA)GTACCACGTGTTACCCATTCTGTGGCCCAGT NGG 10 8 17 8 24 0 100 5 10
    240613123935_kuo7 TGCTACCCAAGGAGAGCATGCCAGATAGCGAG(+AGGACA)GATCCAAGGTCAAGGCAGAGCCTTGACAGCT NGG 10 8 17 8 24 0 100 5 10