Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613123840_n0dn GAGGGAGTGGGATATGTTATGCGGGATCTGGG(+AGGACA)GTACCCTGAAGACCCAGGAGTAAACCAGAGG NGG 10 8 17 8 24 0 100 5 10
    240613123839_1svv TTATACCTCTGGTTTACTCCTGGGTCTTCAGG(+AGGACA)GTACCCCAGATCCCGCATAACATATCCCACT NGG 10 8 17 8 24 0 100 5 10
    240613123837_rs08 ATATGTAAAGGATACACTGTTTGAATGTGCTG(+AGGACA)GTACCTGCTTTGCTTCTGGAGTTGAGCCACC NGG 10 8 17 8 24 0 100 5 10
    240613123836_cwmr GCACTGGTGGCTCAACTCCAGAAGCAAAGCAG(+AGGACA)GTACCAGCACATTCAAACAGTGTATCCTTTA NGG 10 8 17 8 24 0 100 5 10
    240613123834_0nqu AGTAATGGAAGCTTAGCGGCCTGGAGAAACCG(+AGGACA)GATCCAGATATAAGCCAGCGTTCCTGGCAGG NGG 10 8 17 8 24 0 100 5 10
    240613123833_he3w CGGGTCCTGCCAGGAACGCTGGCTTATATCTG(+AGGACA)GATCCGGTTTCTCCAGGCCGCTAAGCTTCCA NGG 10 8 17 8 24 0 100 5 10
    240613123831_e62c AGAGCAAGAGGCTGGACTTGATGGTGCCGTTG(+AGGACA)GATCCCAGGTGCTCGCTCGCGGTTCTCATCC NGG 10 8 17 8 24 0 100 5 10
    240613123829_5nk0 CAAAAGGATGAGAACCGCGAGCGAGCACCTGG(+AGGACA)GATCCAACGGCACCATCAAGTCCAGCCTCTT NGG 10 8 17 8 24 0 100 5 10
    240613123828_c30u GGGAAGCTCATCACATTGTAAGGCTCTCACTG(+AGGACA)GTACCACCCAGTCCTAATCAAGAATGGCATT NGG 10 8 17 8 24 0 100 5 10
    240613123826_t7gb CCAGAAATGCCATTCTTGATTAGGACTGGGTG(+AGGACA)GTACCAGTGAGAGCCTTACAATGTGATGAGC NGG 10 8 17 8 24 0 100 5 10