Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613123810_q7fo GTCCTTGGTTGTGAAGTATCATCCCAGTCATG(+AGGACA)GTACCCTGTGCTGGCAACAGAAAAAAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240613123808_5iao TATTTTTTTTTTTTTTCTGTTGCCAGCACAGG(+AGGACA)GTACCATGACTGGGATGATACTTCACAACCA NGG 10 8 17 8 24 0 100 5 10
    240613123806_vwon AGGCCTCACATCCCCACTCTGCTCCCCGCCTG(+AGGACA)GTACCTGTGTTAATTTAGACCTTCACCACCT NGG 10 8 17 8 24 0 100 5 10
    240613123805_xlgr GAGGCAGGTGGTGAAGGTCTAAATTAACACAG(+AGGACA)GTACCAGGCGGGGAGCAGAGTGGGGATGTGA NGG 10 8 17 8 24 0 100 5 10
    240613123802_lch2 ATAAGTCCCCATTTGAGGTCTAGGTTCCCAGG(+AGGACA)GATCCATTTACCTGGCCAGCCAGAAGCCCCT NGG 10 8 17 8 24 0 100 5 10
    240613123801_ksw3 TATGCAGGGGCTTCTGGCTGGCCAGGTAAATG(+AGGACA)GATCCCTGGGAACCTAGACCTCAAATGGGGA NGG 10 8 17 8 24 0 100 5 10
    240613123759_noq3 CAATAAACTTTCTTGAATCTTCGAGTCTCCTG(+AGGACA)GATCCCCATGGCCTCCATCACATCTTCTTCT NGG 10 8 17 8 24 0 100 5 10
    240613123757_89g2 ACATTAGAAGAAGATGTGATGGAGGCCATGGG(+AGGACA)GATCCAGGAGACTCGAAGATTCAAGAAAGTT NGG 10 8 17 8 24 0 100 5 10
    240613123756_hh1l TTAGATCGATTTTTTTTAAAAGTATGTGTTGG(+AGGACA)GTACCTTTTCTGTGTGTATGCTCAGGCAGGC NGG 10 8 17 8 24 0 100 5 10
    240613123755_j1zz ACACAGCCTGCCTGAGCATACACACAGAAAAG(+AGGACA)GTACCCAACACATACTTTTAAAAAAAATCGA NGG 10 8 17 8 24 0 100 5 10