Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613123711_va3k GGATGGACCATCTAGAGACTGCCATATCCAGG(+AGGACA)GATCCATCCCATAATTAGCCTCCAAAGGATG NGG 10 8 17 8 24 0 100 5 10
    240613123710_258r GGTGTCATCCTTTGGAGGCTAATTATGGGATG(+AGGACA)GATCCCTGGATATGGCAGTCTCTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240613123708_7fz6 ATTAGTAAGTCTAAAACACAAATAATTATAAG(+AGGACA)GATCCGGAGTTTATATCTTTTCAAGAGCTAT NGG 10 8 17 8 24 0 100 5 10
    240613123707_l786 TACAGATAGCTCTTGAAAAGATATAAACTCCG(+AGGACA)GATCCTTATAATTATTTGTGTTTTAGACTTA NGG 10 8 17 8 24 0 100 5 10
    240613123705_pjas TTCTGGATATATGCCCAGGAGAGGTATTGTGG(+AGGACA)GATCCTCCAGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240613123704_qorh TCCTTAGAAAATTGGACATAGTACTACTGGAG(+AGGACA)GATCCCACAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240613123702_i77l GTGTCCTGGATTTCCTGGATGTTTTGAGTTAG(+AGGACA)GATCCTTTTGCATTTTGTATTTTCTTTGATT NGG 10 8 17 8 24 0 100 5 10
    240613123701_qhtl ACAACAATCAAAGAAAATACAAAATGCAAAAG(+AGGACA)GATCCTAACTCAAAACATCCAGGAAATCCAG NGG 10 8 17 8 24 0 100 5 10
    240613123700_k7vc AGACTGAGGGATCAAACCACATGATGCAGTTG(+AGGACA)GATCCCTAAGGCCCCACCTCAGAATACTGTT NGG 10 8 17 8 24 0 100 5 10
    240613123658_g177 AATGCAACAGTATTCTGAGGTGGGGCCTTAGG(+AGGACA)GATCCAACTGCATCATGTGGTTTGATCCCTC NGG 10 8 17 8 24 0 100 5 10