Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613123657_wnj5 CTGGGAGAATGAGGGCCCACTGAACAAGCAGG(+AGGACA)GATCCAATTCACACATCAAAGTCTGTAAAGA NGG 10 8 17 8 24 0 100 5 10
    240613123656_tn06 TCTTCTCTTTACAGACTTTGATGTGTGAATTG(+AGGACA)GATCCCTGCTTGTTCAGTGGGCCCTCATTCT NGG 10 8 17 8 24 0 100 5 10
    240613123654_5w0h AAGAATAAGAAACCAAGAGTCGAGAGATCCAG(+AGGACA)GATCCCATTCTGCGGCTGCTGTTTGCTGAGT NGG 10 8 17 8 24 0 100 5 10
    240613123652_27a1 GGGTTACTCAGCAAACAGCAGCCGCAGAATGG(+AGGACA)GATCCTGGATCTCTCGACTCTTGGTTTCTTA NGG 10 8 17 8 24 0 100 5 10
    240613123650_i3gq CCTTGTGCTCTGTCAGGAGCCATAGCTCCTGG(+AGGACA)GATCCTTAAGATAGAAGTGGGTGACCCGTGT NGG 10 8 17 8 24 0 100 5 10
    240613123649_pnn7 AGTGTACACGGGTCACCCACTTCTATCTTAAG(+AGGACA)GATCCCAGGAGCTATGGCTCCTGACAGAGCA NGG 10 8 17 8 24 0 100 5 10
    240613123647_7p56 AATCTTCTCTACCCAAATCCTCCTCATAAATG(+AGGACA)GATCCATGATGACAGCTAAGAGAAAAAAAAT NGG 10 8 17 8 24 0 100 5 10
    240613123646_novx TACTTATTTTTTTTCTCTTAGCTGTCATCATG(+AGGACA)GATCCATTTATGAGGAGGATTTGGGTAGAGA NGG 10 8 17 8 24 0 100 5 10
    240613123645_az6i TGCAAAGGACTGGAGATACAGGGTTAAGACAG(+AGGACA)GTACCATCTACATATATTCTGAACAATGCAG NGG 10 8 17 8 24 0 100 5 10
    240613123644_992i CTACACTGCATTGTTCAGAATATATGTAGATG(+AGGACA)GTACCTGTCTTAACCCTGTATCTCCAGTCCT NGG 10 8 17 8 24 0 100 5 10