Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613123642_qaok GGGTAATGCAAAGAAAATGAGTTTTCCTTTGG(+AGGACA)GTACCAGTAATTGTCAAAGGAATGATTGCAG NGG 10 8 17 8 24 0 100 5 10
    240613123641_ug5t TGAATCTGCAATCATTCCTTTGACAATTACTG(+AGGACA)GTACCCAAAGGAAAACTCATTTTCTTTGCAT NGG 10 8 17 8 24 0 100 5 10
    240613123640_9rsx GCTGCACTTGAGGATAAATACCGGAGCTGTAG(+AGGACA)GTACCACTGCACCTTGTGTACATGGAACCTA NGG 10 8 17 8 24 0 100 5 10
    240613123638_8kdh ATGGATAGGTTCCATGTACACAAGGTGCAGTG(+AGGACA)GTACCTACAGCTCCGGTATTTATCCTCAAGT NGG 10 8 17 8 24 0 100 5 10
    240613123637_5dlv GAACTAGTCATAAAGAGTGAGGTTGGTGTGAG(+AGGACA)GTACCAGACATGTAAGAAACAAGGGGACACA NGG 10 8 17 8 24 0 100 5 10
    240613123636_4hal GGTGGTGTGTCCCCTTGTTTCTTACATGTCTG(+AGGACA)GTACCTCACACCAACCTCACTCTTTATGACT NGG 10 8 17 8 24 0 100 5 10
    240613123634_pyhx GCACTGCAAGAAACACTACATTCTACACCTGG(+AGGACA)GTACCTGTGTGTGTTCACTGTATTTCTTTGT NGG 10 8 17 8 24 0 100 5 10
    240613123633_nksb TTACCACAAAGAAATACAGTGAACACACACAG(+AGGACA)GTACCCAGGTGTAGAATGTAGTGTTTCTTGC NGG 10 8 17 8 24 0 100 5 10
    240613123631_1zw7 TTCAATTCAGAGCTTTGGGTGTATTTTCTGGG(+AGGACA)GATCCACAGCTTTCTATTCAAGCAAGTTGGG NGG 10 8 17 8 24 0 100 5 10
    240613123630_rjra AATTGCCCAACTTGCTTGAATAGAAAGCTGTG(+AGGACA)GATCCCCAGAAAATACACCCAAAGCTCTGAA NGG 10 8 17 8 24 0 100 5 10