Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613123600_2s7a TTGCGTGCCACCCGCAGCCCCCAACCTTGTAG(+AGGACA)GATCCGGGCGATCCCCATGAGGCTCCCTGAA NGG 10 8 17 8 24 0 100 5 10
    240613123557_gt2a CTGAGTTCAGGGAGCCTCATGGGGATCGCCCG(+AGGACA)GATCCTACAAGGTTGGGGGCTGCGGGTGGCA NGG 10 8 17 8 24 0 100 5 10
    240613123555_phmy CGCCCTCTAGTGACCGGGAGGCCAGTAACAGG(+AGGACA)GATCCCTTTCTCCAAATGACCAAATGTTGGG NGG 10 8 17 8 24 0 100 5 10
    240613123554_s16r ATCGACCCAACATTTGGTCATTTGGAGAAAGG(+AGGACA)GATCCCTGTTACTGGCCTCCCGGTCACTAGA NGG 10 8 17 8 24 0 100 5 10
    240613123552_bsb5 ATTTATACATAAAACTCCCAAGGAACCATGTG(+AGGACA)GATCCGACAGAATGTTAATAGCACATCTAAG NGG 10 8 17 8 24 0 100 5 10
    240613123551_3zib TTATTCTTAGATGTGCTATTAACATTCTGTCG(+AGGACA)GATCCACATGGTTCCTTGGGAGTTTTATGTA NGG 10 8 17 8 24 0 100 5 10
    240613123550_5sez TTCTGTACTGCTTAAGATTCAGCAGGGCACTG(+AGGACA)GATCCCTGGGAGCTGGAGTTAGGTGACTATG NGG 10 8 17 8 24 0 100 5 10
    240613123547_l729 CCACTCATAGTCACCTAACTCCAGCTCCCAGG(+AGGACA)GATCCAGTGCCCTGCTGAATCTTAAGCAGTA NGG 10 8 17 8 24 0 100 5 10
    240613123546_wsz8 GCATCAAGTCTACCTTTTGCATTGGTTTTATG(+AGGACA)GATCCATGTATATAGGTAGCCATCATAAACT NGG 10 8 17 8 24 0 100 5 10
    240613123545_wji3 CCTTCAGTTTATGATGGCTACCTATATACATG(+AGGACA)GATCCATAAAACCAATGCAAAAGGTAGACTT NGG 10 8 17 8 24 0 100 5 10