Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613123459_1yoe TTCTCATTCAGTAAAGGGAACTTGCTACAAAG(+AGGACA)GTACCCAAAACACACTTTCTGTTCTGCCGGT NGG 10 8 17 8 24 0 100 5 10
    240613123457_d3di TGCTGACCGGCAGAACAGAAAGTGTGTTTTGG(+AGGACA)GTACCTTTGTAGCAAGTTCCCTTTACTGAAT NGG 10 8 17 8 24 0 100 5 10
    240613123456_46px AAGGTTCTCACTGTCGTCAACAATCTTTCTAG(+AGGACA)GTACCTTTGGATGGTTCTAGCACATTTATCA NGG 10 8 17 8 24 0 100 5 10
    240613123454_fziy TTGTCTGATAAATGTGCTAGAACCATCCAAAG(+AGGACA)GTACCTAGAAAGATTGTTGACGACAGTGAGA NGG 10 8 17 8 24 0 100 5 10
    240613123453_c789 GAACCCCTGAACTTGGAAAACTGAGCCAAGAG(+AGGACA)GATCCCAAGTTCAAAGCCAGCCTGGGCTACA NGG 10 8 17 8 24 0 100 5 10
    240613123452_et3b TGCTATGTAGCCCAGGCTGGCTTTGAACTTGG(+AGGACA)GATCCTCTTGGCTCAGTTTTCCAAGTTCAGG NGG 10 8 17 8 24 0 100 5 10
    240613123449_wl8k GGGACTGCCTCTAATGTGCGTTTGATTCCCTG(+AGGACA)GTACCTGACACTGTACCCAGTACACACAAAT NGG 10 8 17 8 24 0 100 5 10
    240613123448_sh8v TGTGCATTTGTGTGTACTGGGTACAGTGTCAG(+AGGACA)GTACCAGGGAATCAAACGCACATTAGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240613123446_ytd3 TATATAGACCAGGTCAGGCCTGAATTCACAGG(+AGGACA)GATCCACCTGCCTCTGTCTCCCTGCTGCTGA NGG 10 8 17 8 24 0 100 5 10
    240613123445_0w6o TGATCTCAGCAGCAGGGAGACAGAGGCAGGTG(+AGGACA)GATCCCTGTGAATTCAGGCCTGACCTGGTCT NGG 10 8 17 8 24 0 100 5 10