Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613123356_tzk6 GAGTCAGATTAGTGGTGCTAGGAACTGAGCAG(+AGGACA)GATCCCCAAGTTTATTTTCTAGAACCTTTAA NGG 10 8 17 8 24 0 100 5 10
    240613123354_707o ATTCTTTAAAGGTTCTAGAAAATAAACTTGGG(+AGGACA)GATCCTGCTCAGTTCCTAGCACCACTAATCT NGG 10 8 17 8 24 0 100 5 10
    240613123353_2j35 TATCTCATCTACTCCTGATGTGGGAATCCATG(+AGGACA)GATCCCTGAGCTTTAATTTGTTCTTGCTTGG NGG 10 8 17 8 24 0 100 5 10
    240613123351_pro4 TAAATCCAAGCAAGAACAAATTAAAGCTCAGG(+AGGACA)GATCCATGGATTCCCACATCAGGAGTAGATG NGG 10 8 17 8 24 0 100 5 10
    240613123350_edh7 CCAAAAATAAAAATAAAAAGCTAAACACTATG(+AGGACA)GATCCTTAGCTAGAAAGCACAGAGATGTGGA NGG 10 8 17 8 24 0 100 5 10
    240613123348_jbqe TCACATCCACATCTCTGTGCTTTCTAGCTAAG(+AGGACA)GATCCATAGTGTTTAGCTTTTTATTTTTATT NGG 10 8 17 8 24 0 100 5 10
    240613123346_n6m2 CAACAGGAAGATCAACAGGGTCAACTAACCTG(+AGGACA)GATCCTTGGGGCTCTCAGAGACTGAACCACC NGG 10 8 17 8 24 0 100 5 10
    240613123344_7a9o CGGTTGGTGGTTCAGTCTCTGAGAGCCCCAAG(+AGGACA)GATCCAGGTTAGTTGACCCTGTTGATCTTCC NGG 10 8 17 8 24 0 100 5 10
    240613123342_x2lb TGGGTGGCTTACCTATAATTATAGCTCCAGGG(+AGGACA)GATCCAATCCCCTCTTCTGGCCTCATCAGGC NGG 10 8 17 8 24 0 100 5 10
    240613123340_30e6 TAGGTGCCTGATGAGGCCAGAAGAGGGGATTG(+AGGACA)GATCCCCTGGAGCTATAATTATAGGTAAGCC NGG 10 8 17 8 24 0 100 5 10