Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613123310_454i CAGGTGGCTAACAACTGCCTTTAACTTCAAGG(+AGGACA)GATCCAAAGCCCTCCTCAGGCACCTGTACCC NGG 10 8 17 8 24 0 100 5 10
    240613123309_fvum TATGTGGGTACAGGTGCCTGAGGAGGGCTTTG(+AGGACA)GATCCCTTGAAGTTAAAGGCAGTTGTTAGCC NGG 10 8 17 8 24 0 100 5 10
    240613123308_rs3c AAGTTCTTACAGTTTCCAGACTATAATTTTAG(+AGGACA)GATCCAGAAAGGACTCGGAGAACGCATCCCC NGG 10 8 17 8 24 0 100 5 10
    240613123307_yopo TTGTGGGGGATGCGTTCTCCGAGTCCTTTCTG(+AGGACA)GATCCTAAAATTATAGTCTGGAAACTGTAAG NGG 10 8 17 8 24 0 100 5 10
    240613123305_zz99 AGCCTGTGAGTTATATCCCCCCAGCAGCCATG(+AGGACA)GATCCCATGGCTTCAACAGCGGGTGTACTTG NGG 10 8 17 8 24 0 100 5 10
    240613123303_gdpi ATACACAAGTACACCCGCTGTTGAAGCCATGG(+AGGACA)GATCCATGGCTGCTGGGGGGATATAACTCAC NGG 10 8 17 8 24 0 100 5 10
    240613123301_njuh TCAAAAGACAGGATAAGGTGAAGCCAGAACGG(+AGGACA)GATCCGCTCAGGAAGTCCTGGGGGCACTGGT NGG 10 8 17 8 24 0 100 5 10
    240613123300_2ojz GATGGACCAGTGCCCCCAGGACTTCCTGAGCG(+AGGACA)GATCCCGTTCTGGCTTCACCTTATCCTGTCT NGG 10 8 17 8 24 0 100 5 10
    240613123259_i7w4 TGTCGACCCAGTACTGTGGGCAGAGGCAAGAG(+AGGACA)GATCCCTGCAAAGTGGTGGCCAAACAGAACA NGG 10 8 17 8 24 0 100 5 10
    240613123257_8r54 GGTTTTGTTCTGTTTGGCCACCACTTTGCAGG(+AGGACA)GATCCTCTTGCCTCTGCCCACAGTACTGGGT NGG 10 8 17 8 24 0 100 5 10