Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613123238_lm5n TAAATGTTGTGTTGTTTCTTTCTTCTCAGAGG(+AGGACA)GATCCTGTAATATCACCAAGGCCTTCTTTAT NGG 10 8 17 8 24 0 100 5 10
    240613123237_vcdn CTGGTATAAAGAAGGCCTTGGTGATATTACAG(+AGGACA)GATCCCTCTGAGAAGAAAGAAACAACACAAC NGG 10 8 17 8 24 0 100 5 10
    240613123235_eb0l GCAAATATTTGCTCTTTTCCCAGATTGTCCTG(+AGGACA)GTACCTACCCCTTGTACACATCCCTGCCTAT NGG 10 8 17 8 24 0 100 5 10
    240613123234_q1nq CTTTTATAGGCAGGGATGTGTACAAGGGGTAG(+AGGACA)GTACCAGGACAATCTGGGAAAAGAGCAAATA NGG 10 8 17 8 24 0 100 5 10
    240613123233_k4w3 TTCTTCATGAACGATATTTGACATGTGCTTTG(+AGGACA)GTACCCTTCTCTGAAAGTTGAAAACCTACCT NGG 10 8 17 8 24 0 100 5 10
    240613123231_qfu3 TAAGTAGGTAGGTTTTCAACTTTCAGAGAAGG(+AGGACA)GTACCAAAGCACATGTCAAATATCGTTCATG NGG 10 8 17 8 24 0 100 5 10
    240613123230_glui AGAAAGGGCTGCAGTCAGTTTGACTAAAACGG(+AGGACA)GATCCACGTTATTGCTTAGAAAGGAAGCAAG NGG 10 8 17 8 24 0 100 5 10
    240613123229_938r GCTGGCTTGCTTCCTTTCTAAGCAATAACGTG(+AGGACA)GATCCCGTTTTAGTCAAACTGACTGCAGCCC NGG 10 8 17 8 24 0 100 5 10
    240613123227_e2gs AGTATAGTATCAATAACGTCTACATCACCAGG(+AGGACA)GTACCTTTGCATTAAATGAGTTTTGCAAAAT NGG 10 8 17 8 24 0 100 5 10
    240613123225_rvc2 TTACTATTTTGCAAAACTCATTTAATGCAAAG(+AGGACA)GTACCCTGGTGATGTAGACGTTATTGATACT NGG 10 8 17 8 24 0 100 5 10