Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613123156_a1lp GTAAAAGGTCTACAATACCAGATGCGAGTCGG(+AGGACA)GATCCAAATCACCCAGCCTAACTGTGCTTAG NGG 10 8 17 8 24 0 100 5 10
    240613123155_83ky TGTAGCTAAGCACAGTTAGGCTGGGTGATTTG(+AGGACA)GATCCCGACTCGCATCTGGTATTGTAGACCT NGG 10 8 17 8 24 0 100 5 10
    240613123153_oljr ATGGAGAGGGTGTGCACTAACTGAAACTTTTG(+AGGACA)GATCCAAGGACAAGACAATCTTTTCCTTATC NGG 10 8 17 8 24 0 100 5 10
    240613123151_n94s AAACTGATAAGGAAAAGATTGTCTTGTCCTTG(+AGGACA)GATCCAAAAGTTTCAGTTAGTGCACACCCTC NGG 10 8 17 8 24 0 100 5 10
    240613123150_glho GTGCTAAAGAGATTTTCACAGACATCTTCCAG(+AGGACA)GTACCTGGTCTAGTTAGTGGCAGGGATATGT NGG 10 8 17 8 24 0 100 5 10
    240613123148_w5ji GTAAAACATATCCCTGCCACTAACTAGACCAG(+AGGACA)GTACCTGGAAGATGTCTGTGAAAATCTCTTT NGG 10 8 17 8 24 0 100 5 10
    240613123147_udt4 TCGGTGTGGAAAAAAGATGACCAGAAAGTCAG(+AGGACA)GTACCCCGAATCACTGGAAACTCCTTTCTTA NGG 10 8 17 8 24 0 100 5 10
    240613123145_h9j5 CTACATAAGAAAGGAGTTTCCAGTGATTCGGG(+AGGACA)GTACCTGACTTTCTGGTCATCTTTTTTCCAC NGG 10 8 17 8 24 0 100 5 10
    240613123144_iwar TTTTATTAATGTTGAATATTTTCTATCTAGTG(+AGGACA)GATCCTTGAGAAAGTAATGTCCTACTTGAAT NGG 10 8 17 8 24 0 100 5 10
    240613123143_g6a2 CAATTATTCAAGTAGGACATTACTTTCTCAAG(+AGGACA)GATCCACTAGATAGAAAATATTCAACATTAA NGG 10 8 17 8 24 0 100 5 10