Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613123112_5y63 GATGCACCAGGTGAACCTTGGGCATCTAACTG(+AGGACA)GATCCTCATTTATTACCAAAGATAGTTGGTT NGG 10 8 17 8 24 0 100 5 10
    240613123110_gmnr TATGAAACCAACTATCTTTGGTAATAAATGAG(+AGGACA)GATCCAGTTAGATGCCCAAGGTTCACCTGGT NGG 10 8 17 8 24 0 100 5 10
    240613123109_xjx6 TTTTTTATTTTTAAATTTTTTTTTAAGCACGG(+AGGACA)GATCCTGTCTTCACTCCTACACACTGAACAT NGG 10 8 17 8 24 0 100 5 10
    240613123108_qdq1 TGGATATGTTCAGTGTGTAGGAGTGAAGACAG(+AGGACA)GATCCCGTGCTTAAAAAAAAATTTAAAAATA NGG 10 8 17 8 24 0 100 5 10
    240613123106_iubu GCTGTCTTCAGACACACCAGAAGAGGGCATCG(+AGGACA)GATCCCATTACAGATGGTTGTGAGCAATTGT NGG 10 8 17 8 24 0 100 5 10
    240613123105_cyp5 GCCACACAATTGCTCACAACCATCTGTAATGG(+AGGACA)GATCCGATGCCCTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240613123104_tb5l ACCTTTACAGACTTGCCCCTTGCCTCACACAG(+AGGACA)GATCCAATCAAATTTAATTCAAGTCTCAATG NGG 10 8 17 8 24 0 100 5 10
    240613123103_h11q TTTCACATTGAGACTTGAATTAAATTTGATTG(+AGGACA)GATCCTGTGTGAGGCAAGGGGCAAGTCTGTA NGG 10 8 17 8 24 0 100 5 10
    240613123101_4g4m AATGAGTCAGAATTGGCCCTACAGCCCCTTTG(+AGGACA)GATCCTGCAGGATACCCTAGTGGCTAAACCT NGG 10 8 17 8 24 0 100 5 10
    240613123059_2kv3 GGGCTAGGTTTAGCCACTAGGGTATCCTGCAG(+AGGACA)GATCCAAAGGGGCTGTAGGGCCAATTCTGAC NGG 10 8 17 8 24 0 100 5 10