Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613123043_lykq GCTGAGGGGCTGAGTTCCCTTGAAGCAGCCAG(+AGGACA)GTACCGGTGGCCAGCTCTCTCTTGTTCTAGA NGG 10 8 17 8 24 0 100 5 10
    240613123041_ghnm TGGACTCTAGAACAAGAGAGAGCTGGCCACCG(+AGGACA)GTACCTGGCTGCTTCAAGGGAACTCAGCCCC NGG 10 8 17 8 24 0 100 5 10
    240613123039_tiis CAGCATCACCTGCCCCTACCCAGTGCCCACAG(+AGGACA)GTACCAGTAAACCTTAGGAACACAAAGTCAG NGG 10 8 17 8 24 0 100 5 10
    240613123038_oa0f TGAAGCTGACTTTGTGTTCCTAAGGTTTACTG(+AGGACA)GTACCTGTGGGCACTGGGTAGGGGCAGGTGA NGG 10 8 17 8 24 0 100 5 10
    240613123036_yo71 ATTTTCTCTTTATAACCTTGGTTTTGTTGGAG(+AGGACA)GATCCTCACCTAGAAAGTTGAAGACAGAGAT NGG 10 8 17 8 24 0 100 5 10
    240613123035_3ep3 CATTTATCTCTGTCTTCAACTTTCTAGGTGAG(+AGGACA)GATCCTCCAACAAAACCAAGGTTATAAAGAG NGG 10 8 17 8 24 0 100 5 10
    240613123034_w215 ATAGTAATGACCTTCTTTGAAAATAGCTGTGG(+AGGACA)GTACCCATAAGAGCTTCTGAATGGGATTGTG NGG 10 8 17 8 24 0 100 5 10
    240613123032_osz5 ATGCTCACAATCCCATTCAGAAGCTCTTATGG(+AGGACA)GTACCCACAGCTATTTTCAAAGAAGGTCATT NGG 10 8 17 8 24 0 100 5 10
    240613123029_oi7y GTTTGTAGAGGGACCTTTCTTCCCTTCCCCTG(+AGGACA)GATCCCGAAGGTGACAGGCTGTTGTCTGGAT NGG 10 8 17 8 24 0 100 5 10
    240613123027_k91w CCACCATCCAGACAACAGCCTGTCACCTTCGG(+AGGACA)GATCCAGGGGAAGGGAAGAAAGGTCCCTCTA NGG 10 8 17 8 24 0 100 5 10