Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613122942_dzvl ACAGAGGAAGTGTTCTAGAGATAATCAAGGTG(+AGGACA)GTACCTTGTGCTGTGGCTGGACTTGGTAGTG NGG 10 8 17 8 24 0 100 5 10
    240613122941_1ltz TTACACACTACCAAGTCCAGCCACAGCACAAG(+AGGACA)GTACCACCTTGATTATCTCTAGAACACTTCC NGG 10 8 17 8 24 0 100 5 10
    240613122939_c62d TCTTTTGGAGTTGTCCAAAAGATCGTGAGTTG(+AGGACA)GATCCCAGACATTGGACAGTTGGAGTTTAAT NGG 10 8 17 8 24 0 100 5 10
    240613122938_se0p TAAAAATTAAACTCCAACTGTCCAATGTCTGG(+AGGACA)GATCCAACTCACGATCTTTTGGACAACTCCA NGG 10 8 17 8 24 0 100 5 10
    240613122936_il82 TTACAAACTACCAAGTCCAGCCACAGCACAAG(+AGGACA)GTACCACCTTGATTATCTCTAGAACACTTCC NGG 10 8 17 8 24 0 100 5 10
    240613122935_1j1l ACAGAGGAAGTGTTCTAGAGATAATCAAGGTG(+AGGACA)GTACCTTGTGCTGTGGCTGGACTTGGTAGTT NGG 10 8 17 8 24 0 100 5 10
    240613122933_a5ph GAAAAATTAAATTCCAACTGTCCAACGTCTGG(+AGGACA)GATCCAATTCATGATCTTCTGGGCTCCTCCA NGG 10 8 17 8 24 0 100 5 10
    240613122932_wngt GCCCTTGGAGGAGCCCAGAAGATCATGAATTG(+AGGACA)GATCCCAGACGTTGGACAGTTGGAATTTAAT NGG 10 8 17 8 24 0 100 5 10
    240613122931_0rl5 AACAAAAGGATATACCTTCTCAGCACCTCATG(+AGGACA)GTACCTTCTCCAAAATCGACCAAGAGAACCA NGG 10 8 17 8 24 0 100 5 10
    240613122930_q3rn CTCTTTGGTTCTCTTGGTCGATTTTGGAGAAG(+AGGACA)GTACCATGAGGTGCTGAGAAGGTATATCCTT NGG 10 8 17 8 24 0 100 5 10