Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613122915_6j4k ATAAGTAACATCTATTTGCCATAACTGAGTAG(+AGGACA)GTACCAGTCCTAGAGGATTAACTCCTAGAGG NGG 10 8 17 8 24 0 100 5 10
    240613122914_pnb0 TTAATCCTCTAGGAGTTAATCCTCTAGGACTG(+AGGACA)GTACCTACTCAGTTATGGCAAATAGATGTTA NGG 10 8 17 8 24 0 100 5 10
    240613122913_xtaj TTACACACTACCAAGTCCAGCCACAGCACAAG(+AGGACA)GTACCACCTTGATTATCTCTAGAACACTTCC NGG 10 8 17 8 24 0 100 5 10
    240613122911_9uet ACAGAGGAAGTGTTCTAGAGATAATCAAGGTG(+AGGACA)GTACCTTGTGCTGTGGCTGGACTTGGTAGTG NGG 10 8 17 8 24 0 100 5 10
    240613122910_cecp TAAAAATTAAACTCCAACTGTCCAATGTCTCG(+AGGACA)GATCCAACTCACGATCTTTTGGACAACTCCA NGG 10 8 17 8 24 0 100 5 10
    240613122909_r6bf TCTTTTGGAGTTGTCCAAAAGATCGTGAGTTG(+AGGACA)GATCCGAGACATTGGACAGTTGGAGTTTAAT NGG 10 8 17 8 24 0 100 5 10
    240613122907_12cc ACAGAGGAAGTGTTCTAGAGATAATCAAGGTG(+AGGACA)GTACCTTGTGCTGTGGCTGGACTTGGTAGTG NGG 10 8 17 8 24 0 100 5 10
    240613122906_jfkc TTACACACTACCAAGTCCAGCCACAGCACAAG(+AGGACA)GTACCACCTTGATTATCTCTAGAACACTTCC NGG 10 8 17 8 24 0 100 5 10
    240613122905_nm7r TTAATCCTCTAGGAGTTAATCCTCTAGGACTG(+AGGACA)GTACCTACTCAGTTATGGCAAATAGACGTTA NGG 10 8 17 8 24 0 100 5 10
    240613122904_vcvo ATAAGTAACGTCTATTTGCCATAACTGAGTAG(+AGGACA)GTACCAGTCCTAGAGGATTAACTCCTAGAGG NGG 10 8 17 8 24 0 100 5 10