Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613124615_l32r ATTTGGGTTAGAAATAAACAGCCTGGACTTTG(+AGGACA)GTACCTAACCTACTAAGATGAGTAAGCTACA NGG 10 8 17 8 24 0 100 5 10
    240613124613_p4d4 TGTCATCCCTCTTTGGGCCCGAAGGCCAGAGG(+AGGACA)GTACCAACTGGGCAAATGCCATCTGCCTTTT NGG 10 8 17 8 24 0 100 5 10
    240613124612_8jvl TGGGTAAAAGGCAGATGGCATTTGCCCAGTTG(+AGGACA)GTACCCTCTGGCCTTCGGGCCCAAAGAGGGA NGG 10 8 17 8 24 0 100 5 10
    240613124611_zzqj CCTAGGTTATGAATGACAGCCACAGATGCTGG(+AGGACA)GATCCCTATTGAGAAGGCAGTGCTATCGAGA NGG 10 8 17 8 24 0 100 5 10
    240613124609_be88 CGGGCTCTCGATAGCACTGCCTTCTCAATAGG(+AGGACA)GATCCCAGCATCTGTGGCTGTCATTCATAAC NGG 10 8 17 8 24 0 100 5 10
    240613124607_9uix GGTTGCTCCGTGCATAACTAGTTCTTTCTGGG(+AGGACA)GATCCTGTCTGTCTCTTTTTTCCCCTGACCT NGG 10 8 17 8 24 0 100 5 10
    240613124606_81ds GACAGAGGTCAGGGGAAAAAAGAGACAGACAG(+AGGACA)GATCCCCAGAAAGAACTAGTTATGCACGGAG NGG 10 8 17 8 24 0 100 5 10
    240613124605_f7sy AGTGCCTTTTGAGGTCCAGTCGCTGTGGCTCG(+AGGACA)GATCCATTCATCTCTATGTTACCTCCTACTC NGG 10 8 17 8 24 0 100 5 10
    240613124603_m7wx CAAACGAGTAGGAGGTAACATAGAGATGAATG(+AGGACA)GATCCGAGCCACAGCGACTGGACCTCAAAAG NGG 10 8 17 8 24 0 100 5 10
    240613124602_c4hr GAATCTGCTTCATTATTGTCTCGGCTCAGGTG(+AGGACA)GTACCTGGTGGCTGAGATTTAAGAGTTGGCC NGG 10 8 17 8 24 0 100 5 10