Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613122955_rzz4 GTCTCCCACTCCTTGCTTGTTGCCTTTAGAAG(+AGGACA)GTACCAGGTGGGACTGGGGCTGTGGGGCAAG NGG 10 8 17 8 24 0 100 5 10
    240613122953_k4t1 TCCACCTTGCCCCACAGCCCCAGTCCCACCTG(+AGGACA)GTACCTTCTAAAGGCAACAAGCAAGGAGTGG NGG 10 8 17 8 24 0 100 5 10
    240613122952_3jzy AGGGGAGCTTGTGATTGCTATGCCTTCAACAG(+AGGACA)GATCCCTCAGGGTCTCATCTTGAAGAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240613122951_2h32 AGCCCTCCACTCTTCAAGATGAGACCCTGAGG(+AGGACA)GATCCTGTTGAAGGCATAGCAATCACAAGCT NGG 10 8 17 8 24 0 100 5 10
    240613122949_6rjt GCTTACATTTACTGGCCCTGAAGCTTCCAAAG(+AGGACA)GATCCGCATAAAAACCTAGAGAAAGAATAAG NGG 10 8 17 8 24 0 100 5 10
    240613122948_gx5k CTTCCCTTATTCTTTCTCTAGGTTTTTATGCG(+AGGACA)GATCCTTTGGAAGCTTCAGGGCCAGTAAATG NGG 10 8 17 8 24 0 100 5 10
    240613122947_b341 TCCTAGGAGGCCTTTGTTTTCCCAAATTCAAG(+AGGACA)GATCCAAAAATTCTTCAGGTCAGAAAACTAA NGG 10 8 17 8 24 0 100 5 10
    240613122945_k7i0 AAAGTTTAGTTTTCTGACCTGAAGAATTTTTG(+AGGACA)GATCCTTGAATTTGGGAAAACAAAGGCCTCC NGG 10 8 17 8 24 0 100 5 10
    240613122944_hafq GCTCAATGTTTTTTGGTTGCATCATGCAAGTG(+AGGACA)GTACCAGCTAGAAACAAGGTGTGAAATAAAA NGG 10 8 17 8 24 0 100 5 10
    240613122943_yida AATAGTTTTATTTCACACCTTGTTTCTAGCTG(+AGGACA)GTACCACTTGCATGATGCAACCAAAAAACAT NGG 10 8 17 8 24 0 100 5 10