Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613122823_v97x GCCCTTGGAGGAGCCCAGAAGATCATGAGTTG(+AGGACA)GATCCCAGACGTTGGACAGTTGGAATTTAAT NGG 10 8 17 8 24 0 100 5 10
    240613122822_vpk0 GAAAAATTAAATTCCAACTGTCCAACGTCTGG(+AGGACA)GATCCAACTCATGATCTTCTGGGCTCCTCCA NGG 10 8 17 8 24 0 100 5 10
    240613122820_g6n3 ACAGAGGAAGTGTTCTAGAGATAATCAAGGTG(+AGGACA)GTACCTTGTGCTGTGGCTGGACTTGGTAGTG NGG 10 8 17 8 24 0 100 5 10
    240613122819_v2re TTACACACTACCAAGTCCAGCCACAGCACAAG(+AGGACA)GTACCACCTTGATTATCTCTAGAACACTTCC NGG 10 8 17 8 24 0 100 5 10
    240613122818_wd73 TTAATCCTCTAGGAGTTAATCCTCTAGGACTG(+AGGACA)GTACCTACTCAGTTATGGCAAATAGACGTTA NGG 10 8 17 8 24 0 100 5 10
    240613122817_hih0 ATAAGTAACGTCTATTTGCCATAACTGAGTAG(+AGGACA)GTACCAGTCCTAGAGGATTAACTCCTAGAGG NGG 10 8 17 8 24 0 100 5 10
    240613122815_sr8j GCTCAATGTTTTTTGGTTGCATCATGCAAGTG(+AGGACA)GTACCAGCTAGAAACAAGGTGTGAAATAAAA NGG 10 8 17 8 24 0 100 5 10
    240613122814_2oei AACAGTTTTATTTCACACCTTGTTTCTAGCTG(+AGGACA)GTACCACTTGCATGATGCAACCAAAAAACAT NGG 10 8 17 8 24 0 100 5 10
    240613122813_6f22 TCTTTTGGAGTTGTCCAAAAGATTGTGAGTTG(+AGGACA)GATCCCAGACATTGGACAGTTGGAGTTTAAT NGG 10 8 17 8 24 0 100 5 10
    240613122812_zwlx TAAAAATTAAACTCCAACTGTCCAATGTCTGG(+AGGACA)GATCCAACTCACAATCTTTTGGACAACTCCA NGG 10 8 17 8 24 0 100 5 10