Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613122810_9wmk ACAGAGGAAGTGTTCTAGAGATAATCAAGGTG(+AGGACA)GTACCTTGTGCTGTGGCTGGACTTGGTAGTG NGG 10 8 17 8 24 0 100 5 10
    240613122809_h6we TTACACACTACCAAGTCCAGCCACAGCACAAG(+AGGACA)GTACCACCTTGATTATCTCTAGAACACTTCC NGG 10 8 17 8 24 0 100 5 10
    240613122807_juot TTAATCCTCTAGGAGTTAATCCTCTAGGACTG(+AGGACA)GTACCTACTCAGTTATGGCAAATAGACGTTA NGG 10 8 17 8 24 0 100 5 10
    240613122806_h2hj ATAAGTAACGTCTATTTGCCATAACTGAGTAG(+AGGACA)GTACCAGTCCTAGAGGATTAACTCCTAGAGG NGG 10 8 17 8 24 0 100 5 10
    240613122805_8on6 GCTCAATGTTTTTTGGTTGCATCATGCAAGTG(+AGGACA)GTACCAGCTAGAAACAAGGTGTGAAATAAAA NGG 10 8 17 8 24 0 100 5 10
    240613122804_xcxa AACAGTTTTATTTCACACCTTGTTTCTAGCTG(+AGGACA)GTACCACTTGCATGATGCAACCAAAAAACAT NGG 10 8 17 8 24 0 100 5 10
    240613122802_we1g TCTTTTGGAGTTGTCCAAAAGATTGTGAGTTG(+AGGACA)GATCCCAGACATTGGACAGTTGGAGTTTAAT NGG 10 8 17 8 24 0 100 5 10
    240613122801_vym0 TAAAAATTAAACTCCAACTGTCCAATGTCTGG(+AGGACA)GATCCAACTCACAATCTTTTGGACAACTCCA NGG 10 8 17 8 24 0 100 5 10
    240613122800_b55y TATCTTGTGAACTGTCACTGCCATGGAGCAAG(+AGGACA)GATCCCACCTCAGTGTTGAGACATCAGAGCT NGG 10 8 17 8 24 0 100 5 10
    240613122758_3sw8 GACCCAGCTCTGATGTCTCAACACTGAGGTGG(+AGGACA)GATCCTTGCTCCATGGCAGTGACAGTTCACA NGG 10 8 17 8 24 0 100 5 10