Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613122743_66jq GCCCTTGGAGGAGCCCAAAGGATCGTGAGTTG(+AGGACA)GATCCCATACTTTGGATAGTTGGAGTTTAAT NGG 10 8 17 8 24 0 100 5 10
    240613122742_dq6t CCAAAATTAAACTCCAACTATCCAAAGTATGG(+AGGACA)GATCCAACTCACGATCCTTTGGGCTCCTCCA NGG 10 8 17 8 24 0 100 5 10
    240613122739_1urs TTTTTGGGCTTATTTTGAAGCCACTCCCCAAG(+AGGACA)GATCCACATTTATTCATGAAAGCAGATATCC NGG 10 8 17 8 24 0 100 5 10
    240613122738_sm69 GGTCTGGATATCTGCTTTCATGAATAAATGTG(+AGGACA)GATCCTTGGGGAGTGGCTTCAAAATAAGCCC NGG 10 8 17 8 24 0 100 5 10
    240613122736_vatf AAATTCAATTTCATAGACTACTGCCCAGCTAG(+AGGACA)GATCCAGGTCTCAAGCACACAGATGCCTCGC NGG 10 8 17 8 24 0 100 5 10
    240613122734_vjse AGTTAGCGAGGCATCTGTGTGCTTGAGACCTG(+AGGACA)GATCCTAGCTGGGCAGTAGTCTATGAAATTG NGG 10 8 17 8 24 0 100 5 10
    240613122733_6udb GCCCAAGTTTCTCTATCAGCCACTCCCACCAG(+AGGACA)GATCCAGCCCCTCAGAGGCACTCAGCTCCTC NGG 10 8 17 8 24 0 100 5 10
    240613122731_xzo2 GGACAGAGGAGCTGAGTGCCTCTGAGGGGCTG(+AGGACA)GATCCTGGTGGGAGTGGCTGATAGAGAAACT NGG 10 8 17 8 24 0 100 5 10
    240613122729_w5yh GAGAGGACTCTGAAGCATCCTAGCTGCCCTGG(+AGGACA)GTACCCAGCAACACTGCTAAACGGCTCCTGG NGG 10 8 17 8 24 0 100 5 10
    240613122727_dbsh GTTTGCCAGGAGCCGTTTAGCAGTGTTGCTGG(+AGGACA)GTACCCAGGGCAGCTAGGATGCTTCAGAGTC NGG 10 8 17 8 24 0 100 5 10