Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613122726_nzl2 AGAGGCACTGCCTCTACACTCATAAATTTTTG(+AGGACA)GTACCAAACTTCTTAAAAACCAGATGGTTAA NGG 10 8 17 8 24 0 100 5 10
    240613122725_volp TTTTTTTAACCATCTGGTTTTTAAGAAGTTTG(+AGGACA)GTACCAAAAATTTATGAGTGTAGAGGCAGTG NGG 10 8 17 8 24 0 100 5 10
    240613122723_c3u7 TTTAAAATATAGGTTACCCTGCTACCCTGCTG(+AGGACA)GATCCTGAGCCTGGAGGCCTGCAGATCCATC NGG 10 8 17 8 24 0 100 5 10
    240613122722_nnsi GCCCGGATGGATCTGCAGGCCTCCAGGCTCAG(+AGGACA)GATCCAGCAGGGTAGCAGGGTAACCTATATT NGG 10 8 17 8 24 0 100 5 10
    240613122720_94c2 GGCAACTGGGGGGAGGTGGGGTCAGGAACCCG(+AGGACA)GATCCACCAAGAGGACAGTTCCCAAAGAGGA NGG 10 8 17 8 24 0 100 5 10
    240613122718_l0ua CTCCCTCCTCTTTGGGAACTGTCCTCTTGGTG(+AGGACA)GATCCGGGTTCCTGACCCCACCTCCCCCCAG NGG 10 8 17 8 24 0 100 5 10
    240613122717_mr7b GTGGAGGTGTCTTTAACAAACACTTTACTTTG(+AGGACA)GTACCAGCACTCAGGTGACACGGCTCAGAGG NGG 10 8 17 8 24 0 100 5 10
    240613122716_jqr3 AAGGGCCTCTGAGCCGTGTCACCTGAGTGCTG(+AGGACA)GTACCAAAGTAAAGTGTTTGTTAAAGACACC NGG 10 8 17 8 24 0 100 5 10
    240613122714_180a TCACCATTCCTAACAGAAGCACTGCACTGTTG(+AGGACA)GTACCGAGAAGGGTGGAAGACAGGAGGGGGC NGG 10 8 17 8 24 0 100 5 10
    240613122713_6z3i CCCCCGCCCCCTCCTGTCTTCCACCCTTCTCG(+AGGACA)GTACCAACAGTGCAGTGCTTCTGTTAGGAAT NGG 10 8 17 8 24 0 100 5 10