Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613122655_srfk ACTTTCTATTCCAACCTCCCTCTACACCAGCG(+AGGACA)GTACCCAACCTTCCTCATGCTGCCACCCTTT NGG 10 8 17 8 24 0 100 5 10
    240613122653_i3lw GTACTAAAGGGTGGCAGCATGAGGAAGGTTGG(+AGGACA)GTACCGCTGGTGTAGAGGGAGGTTGGAATAG NGG 10 8 17 8 24 0 100 5 10
    240613122650_phom GAGAGAGAGGGGTGGACTGAACATGGCCGGGG(+AGGACA)GATCCGTGAGACGGAGAGGAAACTGGCACAG NGG 10 8 17 8 24 0 100 5 10
    240613122649_9ede CTATCCTGTGCCAGTTTCCTCTCCGTCTCACG(+AGGACA)GATCCCCCGGCCATGTTCAGTCCACCCCTCT NGG 10 8 17 8 24 0 100 5 10
    240613122647_ch1i TTAAAGAGTAACATCTACCTTGCAGTGGCCAG(+AGGACA)GATCCTAGGACCTCTCACTGACTAGAGCGCT NGG 10 8 17 8 24 0 100 5 10
    240613122645_feqn ATCAAAGCGCTCTAGTCAGTGAGAGGTCCTAG(+AGGACA)GATCCTGGCCACTGCAAGGTAGATGTTACTC NGG 10 8 17 8 24 0 100 5 10
    240613122644_b2he GCCAGGATCTCTGCTTTTTCCCTTTGCATCCG(+AGGACA)GTACCCCTAACAAAGATTTGTTTCTGCACTA NGG 10 8 17 8 24 0 100 5 10
    240613122642_1z53 TGAAATAGTGCAGAAACAAATCTTTGTTAGGG(+AGGACA)GTACCGGATGCAAAGGGAAAAAGCAGAGATC NGG 10 8 17 8 24 0 100 5 10
    240613122640_z4k4 TGTTTCCTCTGACTTGTTGCACCTGATTTTAG(+AGGACA)GATCCAAAACTTGTTTTGTTGTTACTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240613122639_5hab AAAAGAAAGAAGTAACAACAAAACAAGTTTTG(+AGGACA)GATCCTAAAATCAGGTGCAACAAGTCAGAGG NGG 10 8 17 8 24 0 100 5 10