Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613122553_evsu TGAAAGTATTAGAGGCTGGAGGGACATTATTG(+AGGACA)GTACCCTAAGACGTACCCCATGTTTAGCAGA NGG 10 8 17 8 24 0 100 5 10
    240613122551_ea9u TTTAATCTGCTAAACATGGGGTACGTCTTAGG(+AGGACA)GTACCAATAATGTCCCTCCAGCCTCTAATAC NGG 10 8 17 8 24 0 100 5 10
    240613122549_5fm8 CTGATTGACTAAAGTAGCTGGAAAAGGTTGGG(+AGGACA)GATCCGTGTTTATTAGAAACAGTCCAACAAG NGG 10 8 17 8 24 0 100 5 10
    240613122548_2qa2 CCTTTCTTGTTGGACTGTTTCTAATAAACACG(+AGGACA)GATCCCCAACCTTTTCCAGCTACTTTAGTCA NGG 10 8 17 8 24 0 100 5 10
    240613122546_67dn GGTGAATCGGAAAGGGTGAGAGCAGGGAGGGG(+AGGACA)GATCCCTGGCAGAGCCGTAATATTCCTATGT NGG 10 8 17 8 24 0 100 5 10
    240613122544_6ikh CAGGGACATAGGAATATTACGGCTCTGCCAGG(+AGGACA)GATCCCCCTCCCTGCTCTCACCCTTTCCGAT NGG 10 8 17 8 24 0 100 5 10
    240613122543_qrwg TTTTAAACCATCAAGTGGTGCTGGGGTGTGAG(+AGGACA)GATCCCTCCAACCCCCGGTGACTATAATAGG NGG 10 8 17 8 24 0 100 5 10
    240613122541_3syh GTGAGCCTATTATAGTCACCGGGGGTTGGAGG(+AGGACA)GATCCTCACACCCCAGCACCACTTGATGGTT NGG 10 8 17 8 24 0 100 5 10
    240613122540_8h90 TATGGATATTAGTCACAGCTCTCCCTGGATAG(+AGGACA)GATCCATTGACCGTCTTGTTCTAACAACTTG NGG 10 8 17 8 24 0 100 5 10
    240613122539_0d4r ATTTCCAAGTTGTTAGAACAAGACGGTCAATG(+AGGACA)GATCCTATCCAGGGAGAGCTGTGACTAATAT NGG 10 8 17 8 24 0 100 5 10